Fibrinolytic agents inhibit platelet adhesion onto collagen type I-coated surfaces at high blood flow conditions
| Autor: | Alevriadou Br, Dionne A. Graham, Nelson Ld, Huang Tc |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Platelet Aggregation
Plasmin Platelet Membrane Glycoproteins Platelet membrane glycoprotein chemistry.chemical_compound Platelet Adhesiveness Fibrinolytic Agents Von Willebrand factor Platelet adhesiveness von Willebrand Factor Image Processing Computer-Assisted medicine Humans Streptokinase Platelet Ristocetin Microscopy Video biology Chemistry Fibrinogen Hematology General Medicine Urokinase-Type Plasminogen Activator Adenosine Diphosphate Perfusion Biochemistry Depression Chemical Hemorheology biology.protein Biophysics Platelet aggregation inhibitor Collagen Platelet Aggregation Inhibitors Fibrinolytic agent medicine.drug |
| Zdroj: | Blood Coagulation & Fibrinolysis. 9:213-226 |
| ISSN: | 0957-5235 |
| Popis: | The effect of fibrinolytic agents on platelet adhesion onto insolubilized collagen type I was evaluated. Normal human whole blood samples were incubated with agents and perfused over collagen-coated surfaces in a parallel-plate flow chamber. Platelet adhesion and aggregation were analyzed by video microscopy and image processing. When blood was perfused at 1500/s, both streptokinase and urokinase, each at 500 U/ml, caused a significantly less normalized platelet deposition, compared with controls. At 480/s, platelet deposition was not different between controls and test samples. Inhibition of platelet deposition at high flow rates was partly due to inhibition of platelet adhesion. Both ristocetin- and ADP-induced platelet aggregation were inhibited in test samples. The agents caused proteolytic degradation of plasma fibrinogen, but no degradation of platelet glycoproteins Ib and IIb-IIIa (GPIb and GPIIb-IIIa) and of plasma von Willebrand factor in test samples prior to perfusion. Post-perfusion von Willebrand factor degradation was not found. Plasmin may cause functional changes to plasma proteins and/or platelet receptors, altering their adhesive properties under flow. At high shear, fibrinogen degradation products may interfere with GPIIb-IIIa binding to insolubilized von Willebrand factor, leading to decreased platelet adhesion. Inhibition of platelet adhesion by thrombolytic agents could help maintain vessel patency after recanalization in stenosed arteries. Publishers. |
| Databáze: | OpenAIRE |
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