Glioblastoma-infiltrated innate immune cells resemble M0 macrophage phenotype
| Autor: | Sujit S. Prabhu, Jeffrey S. Weinberg, Nasser K. Yaghi, Ganesh Rao, Ahmed Elakkad, Luke M. Healy, Qianghu Wang, Krishna P. Bhat, Ravesanker Ezhilarasan, Rivka R. Colen, Jun Wei, Benjamin Rodriguez, Gregory N. Fuller, Konrad Gabrusiewicz, Lauren A. Langford, Yuuri Hashimoto, Sourindra Maiti, Brandon D. Liebelt, Wei Li, Laurence J.N. Cooper, Shouhao Zhou, Raymond Sawaya, Amit Bar-Or, Ginu Thomas, Erik P. Sulman, Michael A. Curran, Amy B. Heimberger, Janet M. Bruner, Neal Huang, Jack P. Antel |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell biology Innate immune system medicine.diagnostic_test Microglia Microarray analysis techniques lcsh:R lcsh:Medicine General Medicine Biology Phenotype 3. Good health Flow cytometry Gene expression profiling 03 medical and health sciences 030104 developmental biology Immune system medicine.anatomical_structure Integrin alpha M Immunology Cancer research medicine biology.protein Research Article |
| Zdroj: | JCI Insight, Vol 1, Iss 2 (2016) |
| ISSN: | 2379-3708 |
| Popis: | Glioblastomas are highly infiltrated by diverse immune cells, including microglia, macrophages, and myeloid-derived suppressor cells (MDSCs). Understanding the mechanisms by which glioblastoma-associated myeloid cells (GAMs) undergo metamorphosis into tumor-supportive cells, characterizing the heterogeneity of immune cell phenotypes within glioblastoma subtypes, and discovering new targets can help the design of new efficient immunotherapies. In this study, we performed a comprehensive battery of immune phenotyping, whole-genome microarray analysis, and microRNA expression profiling of GAMs with matched blood monocytes, healthy donor monocytes, normal brain microglia, nonpolarized M0 macrophages, and polarized M1, M2a, M2c macrophages. Glioblastoma patients had an elevated number of monocytes relative to healthy donors. Among CD11b+ cells, microglia and MDSCs constituted a higher percentage of GAMs than did macrophages. GAM profiling using flow cytometry studies revealed a continuum between the M1- and M2-like phenotype. Contrary to current dogma, GAMs exhibited distinct immunological functions, with the former aligned close to nonpolarized M0 macrophages. |
| Databáze: | OpenAIRE |
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