Plasmin on adherent cells: from microvesiculation to apoptosis.: Cell activation by plasmin: microvesiculation, apoptosis

Autor: Laurent Plawinski, Denis Vivien, Eduardo Anglés-Cano, Didier Goux, Loïc Doeuvre
Přispěvatelé: Sérine protéases et physiopathologie de l'unité neurovasculaire, Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre-Imagerie, Neurosciences, et Application aux Pathologies (CI-NAPS - UMR 6232), Normandie Université (NU)-Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Interactions Cellules Organismes Environnement (ICORE), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), Inserm, European Community's Seventh Framework Programme (FP7/2007-2013) under grant agreement [201024]., European Project: 201024,EC:FP7:HEALTH,FP7-HEALTH-2007-A,ARISE(2008), CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)
Jazyk: angličtina
Rok vydání: 2010
Předmět:
genetic structures
Plasmin
Cell
MESH: Cricetinae
MESH: Fibrinolysin
Biochemistry
Tissue plasminogen activator
Cell membrane
0302 clinical medicine
MESH: Cricetulus
Cricetinae
MESH: Tissue Plasminogen Activator
MESH: Plasminogen
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
MESH: Animals
Fibrinolysin
MESH: In Situ Nick-End Labeling
MESH: Blood Platelets
microparticles
0303 health sciences
MESH: Kinetics
apoptosis
Life Sciences
Cell biology
medicine.anatomical_structure
MESH: Cell Survival
Tissue Plasminogen Activator
Cell activation
medicine.drug
Blood Platelets
Cell Survival
Blotting
Western
CHO Cells
MESH: Microscopy
Electron
Biology
Article
MESH: Cell Adhesion
03 medical and health sciences
Cricetulus
MESH: CHO Cells
Cell Adhesion
In Situ Nick-End Labeling
medicine
Animals
Humans
MESH: Blotting
Western
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Cell adhesion
Molecular Biology
030304 developmental biology
MESH: Humans
electron microscopy
MESH: Apoptosis
Plasminogen
Cell Biology
Kinetics
Microscopy
Electron
membrane blebbing
Apoptosis
Plasminogen activator
030217 neurology & neurosurgery
Zdroj: Biochemical Journal
Biochemical Journal, Portland Press, 2010, 432 (2), pp.365-373. 〈10.1042/BJ20100561〉
Biochemical Journal; Vol 432
Biochemical Journal, Portland Press, 2010, 432 (2), pp.365-73. ⟨10.1042/BJ20100561⟩
Biochemical Journal, 2010, 432 (2), pp.365-73. ⟨10.1042/BJ20100561⟩
Biochemical Journal, Portland Press, 2010, 432 (2), pp.365-373. ⟨10.1042/BJ20100561⟩
ISSN: 0264-6021
1470-8728
DOI: 10.1042/BJ20100561〉
Popis: International audience; Cell activation by stressors is characterized by a sequence of detectable phenotypic cell changes. A given stimulus, depending on its strength, induces modifications in the activity of membrane phospholipid transporters and calpains, which lead to phosphatidylserine exposure, membrane blebbing and the release of microparticles (nanoscale membrane vesicles). This vesiculation could be considered as a warning signal that may be followed, if the stimulus is maintained, by cell detachment-induced apoptosis. In the present study, plasminogen incubated with adherent cells is converted into plasmin by constitutively expressed tPA (tissue-type plasminogen activator) or uPA (urokinase-type plasminogen activator). Plasmin formed on the cell membrane then induces a unique response characterized by membrane blebbing and vesiculation. Hitherto unknown for plasmin, these membrane changes are similar to those induced by thrombin on platelets. If plasmin formation persists, matrix proteins are then degraded, cells lose their attachments and enter the apoptotic process, characterized by DNA fragmentation and specific ultrastructural features. Since other proteolytic or inflammatory stimuli may evoke similar responses in different types of adherent cells, the proposed experimental procedure can be used to distinguish activated adherent cells from cells entering the apoptotic process. Such a distinction is crucial for evaluating the effects of mediators, inhibitors and potential therapeutic agents.
Databáze: OpenAIRE
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