Gastric healing effect of p-coumaric acid isolated from Baccharis dracunculifolia DC on animal model
| Autor: | Anny Caroline de Bassi, Larissa Venzon, Luisa Mota da Silva, Mariane Meurer, Philipe Costa, Luana Gouveia, Caroline Arruda, Viviane Miranda Bispo Steimbach, Priscila de Souza, Luísa Nathália Bolda Mariano, Sérgio Faloni de Andrade, Marília Oliveira de Almeida, Jairo Kenupp Bastos, Thaise Boeing, Tauani Caroline Santos França |
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| Rok vydání: | 2020 |
| Předmět: |
Coumaric Acids
Cell Survival Pharmacology medicine.disease_cause 030226 pharmacology & pharmacy p-Coumaric acid Cell Line Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Baccharis dracunculifolia Gastric mucosa medicine Animals Humans MTT assay Stomach Ulcer Acetic Acid Cell Proliferation Peroxidase biology Chemistry Superoxide Dismutase General Medicine Glutathione biology.organism_classification Anti-Ulcer Agents Catalase digestive system diseases Rats Baccharis Disease Models Animal medicine.anatomical_structure Gastric Mucosa Toxicity biology.protein 030211 gastroenterology & hepatology Female Oxidative stress Phytotherapy |
| Zdroj: | Naunyn-Schmiedeberg's archives of pharmacology. 394(1) |
| ISSN: | 1432-1912 |
| Popis: | The p-coumaric acid is a phenolic compound present in large quantities in the extract of Baccharis dracunculifolia DC, a Brazilian medicinal plant used to treat gastric ulcer. Given the necessity for finding new chemical components capable of accelerating gastric healing, in this study, the effects of the p-coumaric acid were evaluated in the acetic acid–induced ulcer model in rats, where histological, inflammatory, and oxidative parameters were analyzed. The healing property was also evaluated in the scratch assay on fibroblast cells (L929) and the cytotoxicity of p-coumaric acid was assessed in both L929 and human gastric adenocarcinoma (AGS) cells by MTT assay. The treatment with p-coumaric acid (10 mg/kg, p.o.) for 7 days, twice a day, decreased by 44.6% the acetic acid–induced gastric ulcer compared with the vehicle-treated group. The vehicle control–treated group showed a larger extension of the ulcer base and an extensive damage into the mucosa and submucosa layers, which were mitigated by the treatment with p-coumaric acid. This beneficial effect was also associated with increased levels of mucin and reduced glutathione, decreased amount of lipid hydroperoxides, and increased superoxide dismutase and catalase activities without interfering with the activity of myeloperoxidase in the gastric tissue. The compound promoted the restructuring of the cell monolayer in the scratch test and did not show toxicity in the L929 cell line, while reduced the viability of the AGS, a lineage of human gastric adenocarcinoma. Thus, p-coumaric acid may be considered a natural source for the treatment of gastric ulcers, by reinforcing protective factors of gastric mucosa and by accelerating gastric healing. |
| Databáze: | OpenAIRE |
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