Additional file 1 of Modulated nanowire scaffold for highly efficient differentiation of mesenchymal stem cells

Autor: Perez, Jose E., Bajaber, Bashaer, Alsharif, Nouf, Martínez-Banderas, Aldo I., Patel, Niketan, Sharip, Ainur, Di Fabrizio, Enzo, Merzaban, Jasmeen, Kosel, Jürgen
Rok vydání: 2022
DOI: 10.6084/m9.figshare.20088005.v1
Popis: Additional file 1: Figure S1. Immunofluorescence staining of CD105 and CD73 on MSCs cultured on Fe-NWs. MSCs were cultured on Fe-NWs or on tissue culture treated plastic (NC, negative control) for the indicated times (2 days or 1 week), and subsequently stained for the MSC stem cell markers, CD105 (green) and CD73 (red). These images are representative images of n = 2 independent experiments. Figure S2. Immunofluorescence staining of CD105 and CD73 on MSCs cultured on magnetically activated Fe NWs. MSCs were cultured on magnetically activated Fe-NWs or on tissue culture treated plastic (NC negative control) for the indicated times (2 days or 1 week), and subsequently stained for the MSC stem cell markers, CD105 (green) and CD73 (red). The NWs and NC were exposed to a magnetic field with an intensity of 250 mT and a frequency of 0.1 Hz that was applied for 12 h per day. These images are representative images of n = 2 independent experiments.
Databáze: OpenAIRE