Brain-Derived Neurotrophic Factor Improves Long-Term Potentiation and Cognitive Functions after Transient Forebrain Ischemia in the Rat
| Autor: | Stefan Schwab, Jürgen Sandkühler, Matthias Spranger, Irina Kiprianova, Siegfried Hoyer |
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| Rok vydání: | 1999 |
| Předmět: |
Male
medicine.medical_specialty Long-Term Potentiation Ischemia Hippocampus Hippocampal formation Neurotransmission Cerebral Ventricles Cognition Prosencephalon Developmental Neuroscience Memory Reference Values Neurotrophic factors Internal medicine Avoidance Learning medicine Animals Infusions Parenteral Rats Wistar Brain-derived neurotrophic factor Brain-Derived Neurotrophic Factor Pyramidal Cells Excitatory Postsynaptic Potentials Long-term potentiation medicine.disease Electric Stimulation Rats Endocrinology nervous system Neurology Ischemic Attack Transient Anesthesia Excitatory postsynaptic potential Psychology |
| Zdroj: | Experimental Neurology. 159:511-519 |
| ISSN: | 0014-4886 |
| DOI: | 10.1006/exnr.1999.7109 |
| Popis: | We investigated the effect of brain-derived neurotrophic factor (BDNF) on hippocampal long-term potentiation (LTP) and cognitive functions after global cerebral ischemia in the rat. After four-vessel occlusion, BDNF was administered via an osmotic minipump continuously over 14 days intracerebroventricularly. Electrophysiological experiments were performed 14 days after cerebral ischemia. Test stimuli and tetanization were delivered to the Schaffer collaterals of the hippocampus and field excitatory postsynaptic potentials (fEPSP) were recorded in the CA1 region. Cognitive impairment was analyzed repeatedly with a passive avoidance test, a hole-board test, and with an activity center on the same animal. In sham-operated animals, LTP was consistantly induced after delivering a tetanus (increase of initial slope of fEPSP to 173 ± 12% of baseline; n = 6). After transient forebrain ischemia LTP could not be induced (117 ± 4% of baseline; n = 7). In ischemic animals treated with BDNF, LTP could be induced (168 ± 28% of baseline; n = 8). Transient forebrain ischemia resulted in a significant decrease in spatial discrimination performance but not of associative memory. The ratios for working memory (WM) and reference memory (RM) 15 days after ischemia were lower in the ischemic rats ( n = 10) than in the sham-operated control animals ( n = 10; WM: 22 ± 6 vs 72 ± 7; RM: 30 ± 7 vs 72 ± 5). Postischemic intracerebroventricular BDNF infusion increased both WM (63 ± 4; n = 10) and RM (58 ± 5; n = 10). The spontaneous locomotor activity did not differ significantly in the three groups. These data indicate a protective effect of BDNF for synaptic transmission and cognitive functions after transient forebrain ischemia. |
| Databáze: | OpenAIRE |
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