Effects of neuritin on the differentiation of bone marrow-derived mesenchymal stem cells into neuron-like cells
| Autor: | Haiyan Wang, Yuanyuan Li, Jinli Zhang, Qian Wang, Dongzheng Li, Jin Huang, Xiaohua Tan, Jingling Zhu, Lei Yang, Pingping Meng |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Cancer Research Cellular differentiation Cell MAP2 neuritin Biology Biochemistry Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine NSE Neurotrophic factors Genetics medicine Animals neurodegenerative diseases Cell Shape neuronal differentiation Molecular Biology Cells Cultured Neurons mesenchymal stem cells neuron-like cells Oncogene Neuropeptides Mesenchymal stem cell Cell Differentiation Articles Cell cycle Cell biology 030104 developmental biology medicine.anatomical_structure Oncology Cancer research Molecular Medicine Female Bone marrow Neuron cell therapy 030217 neurology & neurosurgery |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2017.6987 |
| Popis: | While the neurotrophic factor neuritin is known to be involved in neurodevelopment, the effects of this compound on cell differentiation remain unclear. The present study demonstrated that neuritin treatment induced the differentiation of rat bone marrow-derived mesenchymal stem cells (rBM-MSCs) into neuron-like (NL) cells. For these analyses, rBM-MSCs were incubated with 0.5 µg/ml neuritin for 24 h. Following induction, 27% of the rBM-MSCs exhibited typical NL cell morphologies. Subsequently, NL cells were characterized by examining the expression of neuronal markers and by analysis of cell functions. The findings demonstrated that the NL cells produced by neuritin treatment expressed the neuronal markers neuron-specific enolase and microtubule associate protein 2, and secreted the neurotransmitter 5-hydroxytryptamine. Furthermore, the NL cells exhibited certain partial neural-electrophysiological functions. In conclusion, neuritin treatment may be an effective method for inducing the differentiation of BM-MSCs towards NL cells. This may provide an alternative, potentially complementary tool for disease modeling and the development of cell-based therapies. |
| Databáze: | OpenAIRE |
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