Pesticide-tolerant bacteria isolated from a biopurification system to remove commonly used pesticides to protect water resources
Autor: | F. Gallardo, Bárbara Leiva, Pamela Donoso-Piñol, Heidi Schalchli, Claudio Lamilla, Marcela Levio, Gabriela Briceño, María Cristina Diez |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Achromobacter 010501 environmental sciences 01 natural sciences Biochemistry chemistry.chemical_compound Agricultural Soil Science Metabolites Soil Pollutants Food science Soil Microbiology Multidisciplinary Iprodione biology Pseudomonas Agriculture Nucleic acids Actinobacteria Biodegradation Environmental Ribosomal RNA Water Resources Medicine Chlorpyrifos Proteobacteria Agrochemicals Soil microbiology Research Article Cell biology Cellular structures and organelles Firmicutes Science 030106 microbiology Soil Science 03 medical and health sciences Pesticides Arthrobacter Non-coding RNA 0105 earth and related environmental sciences Bacteria Hydantoins Ecology and Environmental Sciences Organisms Biology and Life Sciences biology.organism_classification Aminoimidazole Carboxamide Metabolism chemistry RNA Pest Control Rhodococcus Ribosomes |
Zdroj: | PLoS ONE PLoS ONE, Vol 15, Iss 6, p e0234865 (2020) |
ISSN: | 1932-6203 |
Popis: | In this study, we selected and characterized different pesticide-tolerant bacteria isolated from a biomixture of a biopurification system that had received continuous applications of a pesticides mixture. The amplicon analysis of biomixture reported that the phyla Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria were predominant. Six strains grew in the presence of chlorpyrifos and iprodione. Biochemical characterization showed that all isolates were positive for esterase, acid phosphatase, among others, and they were identified as Pseudomonas, Rhodococcus and Achromobacter based on molecular and proteomic analysis. Bacterial growth decreased as both pesticide concentrations increased from 10 to 100 mg L-1 in liquid culture. The Achromobacter sp. strain C1 showed the best chlorpyrifos removal rate of 0.072-0.147 d-1 a half-life of 4.7-9.7 d and a maximum metabolite concentration of 2.10 mg L-1 at 120 h. On the other hand, Pseudomonas sp. strain C9 showed the highest iprodione removal rate of 0.100-0.193 d-1 a half-life of 4-7 d and maximum metabolite concentration of 0.95 mg L-1 at 48 h. The Achromobacter and Pseudomonas strains showed a good potential as chlorpyrifos and iprodione-degrading bacteria. |
Databáze: | OpenAIRE |
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