Pesticide-tolerant bacteria isolated from a biopurification system to remove commonly used pesticides to protect water resources

Autor: F. Gallardo, Bárbara Leiva, Pamela Donoso-Piñol, Heidi Schalchli, Claudio Lamilla, Marcela Levio, Gabriela Briceño, María Cristina Diez
Rok vydání: 2020
Předmět:
0301 basic medicine
Achromobacter
010501 environmental sciences
01 natural sciences
Biochemistry
chemistry.chemical_compound
Agricultural Soil Science
Metabolites
Soil Pollutants
Food science
Soil Microbiology
Multidisciplinary
Iprodione
biology
Pseudomonas
Agriculture
Nucleic acids
Actinobacteria
Biodegradation
Environmental
Ribosomal RNA
Water Resources
Medicine
Chlorpyrifos
Proteobacteria
Agrochemicals
Soil microbiology
Research Article
Cell biology
Cellular structures and organelles
Firmicutes
Science
030106 microbiology
Soil Science
03 medical and health sciences
Pesticides
Arthrobacter
Non-coding RNA
0105 earth and related environmental sciences
Bacteria
Hydantoins
Ecology and Environmental Sciences
Organisms
Biology and Life Sciences
biology.organism_classification
Aminoimidazole Carboxamide
Metabolism
chemistry
RNA
Pest Control
Rhodococcus
Ribosomes
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 6, p e0234865 (2020)
ISSN: 1932-6203
Popis: In this study, we selected and characterized different pesticide-tolerant bacteria isolated from a biomixture of a biopurification system that had received continuous applications of a pesticides mixture. The amplicon analysis of biomixture reported that the phyla Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria were predominant. Six strains grew in the presence of chlorpyrifos and iprodione. Biochemical characterization showed that all isolates were positive for esterase, acid phosphatase, among others, and they were identified as Pseudomonas, Rhodococcus and Achromobacter based on molecular and proteomic analysis. Bacterial growth decreased as both pesticide concentrations increased from 10 to 100 mg L-1 in liquid culture. The Achromobacter sp. strain C1 showed the best chlorpyrifos removal rate of 0.072-0.147 d-1 a half-life of 4.7-9.7 d and a maximum metabolite concentration of 2.10 mg L-1 at 120 h. On the other hand, Pseudomonas sp. strain C9 showed the highest iprodione removal rate of 0.100-0.193 d-1 a half-life of 4-7 d and maximum metabolite concentration of 0.95 mg L-1 at 48 h. The Achromobacter and Pseudomonas strains showed a good potential as chlorpyrifos and iprodione-degrading bacteria.
Databáze: OpenAIRE
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