Functional Characterization of Core Components of the Bacillus subtilis Cyclic-Di-GMP Signaling Pathway
| Autor: | Sampriti Mukherjee, Xiaohui Gao, Charles E. Dann, Loubna A. Hammad, Paige M. Matthews, Daniel B. Kearns |
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| Rok vydání: | 2013 |
| Předmět: |
Cyclic di-GMP
Motility Swarming motility Virulence Bacillus subtilis Microbiology chemistry.chemical_compound Bacterial Proteins Amino Acid Sequence Cloning Molecular Cyclic GMP Molecular Biology Molecular Structure biology Biofilm Phosphodiesterase Gene Expression Regulation Bacterial biology.organism_classification Biochemistry chemistry Commentary Signal transduction Gene Deletion Protein Binding Signal Transduction |
| Zdroj: | Journal of Bacteriology. 195:4782-4792 |
| ISSN: | 1098-5530 0021-9193 |
| Popis: | Bis-(3′-5′)-cyclic dimeric GMP (c-di-GMP) is an intracellular second messenger that regulates adaptation processes, including biofilm formation, motility, and virulence in Gram-negative bacteria. In this study, we have characterized the core components of a c-di-GMP signaling pathway in the model Gram-positive bacterium Bacillus subtilis . Specifically, we have directly identified and characterized three active d i g uanylate c yclases, DgcP, DgcK, and DgcW (formerly YtrP, YhcK, and YkoW, respectively), one active c-di-GMP p hospho d i e sterase, PdeH (formerly YuxH), and a cyclic- d i g uanylate (c-di-GMP) r eceptor, DgrA (formerly YpfA). Furthermore, elevation of c-di-GMP levels in B. subtilis led to inhibition of swarming motility, whereas biofilm formation was unaffected. Our work establishes paradigms for Gram-positive c-di-GMP signaling, and we have shown that the concise signaling system identified in B. subtilis serves as a powerful heterologous host for the study of c-di-GMP enzymes from bacteria predicted to possess larger, more-complex signaling systems. |
| Databáze: | OpenAIRE |
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