Antiviral potential of 3′-sialyllactose- and 6′-sialyllactose-conjugated dendritic polymers against human and avian influenza viruses
| Autor: | Thierry Hennet, Sira C. Günther, Nikita M. Podvalnyy, Janine Vetter, Nikolay Khanzhin, Julian D Maier, Silke Stertz |
|---|---|
| Přispěvatelé: | University of Zurich, Stertz, Silke |
| Rok vydání: | 2020 |
| Předmět: |
10028 Institute of Medical Virology
Dendrimers Hemagglutination Inhibition Tests lcsh:Medicine Oligosaccharides Lactose 610 Medicine & health Chick Embryo 02 engineering and technology Conjugated system medicine.disease_cause Antiviral Agents Article Neutralization Virus Madin Darby Canine Kidney Cells 10052 Institute of Physiology Birds 03 medical and health sciences Dogs Species Specificity Dendrimer medicine Animals Humans lcsh:Science Receptor 030304 developmental biology 1000 Multidisciplinary 0303 health sciences Multidisciplinary Hemagglutination assay Chemistry lcsh:R Antivirals 021001 nanoscience & nanotechnology Virology Influenza A virus subtype H5N1 3. Good health Influenza A virus 570 Life sciences biology lcsh:Q Influenza virus 0210 nano-technology |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-9 (2020) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-57608-4 |
| Popis: | Current treatment options for influenza virus infections in humans are limited and therefore the development of novel antivirals is of high priority. Inhibiting influenza virus attachment to host cells would provide an early and efficient block of the infection and thus, receptor analogs have been considered as options for antiviral treatment. Here, we describe the rapid and efficient synthesis of PAMAM dendrimers conjugated with either 3′-sialyllactose (3SL) or 6′-sialyllactose (6SL) and their potential to inhibit a diverse range of human and avian influenza virus strains. We show in a hemagglutination inhibition (HAI) assay that human IAV strains can be inhibited by (6SL)- and to a lesser extent also by (3SL)-conjugated PAMAM dendrimers. In contrast, avian strains could only be inhibited by (3SL)-conjugated dendrimers. Importantly, the differential sensitivities of human and avian IAV to the two types of sialyllactose-conjugated dendrimers could be confirmed in cell-based neutralization assays. Based on our findings, we suggest to further develop both, (3SL)- and (6SL)-conjugated PAMAM dendrimers, as influenza virus inhibitors. |
| Databáze: | OpenAIRE |
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