The Capsular Polysaccharide Vi from Salmonella Typhi Is a B1b Antigen
Autor: | Robert A. Kingsley, Jeremy H. Lakey, Ian R. Henderson, Ewan A. Ross, Adriana Flores-Langarica, Calman A. MacLennan, Jessica Hitchcock, Kai-Michael Toellner, Laura B. Martin, Jennifer L. Marshall, Adam F. Cunningham, Constantino López-Macías, Gordon Dougan, Ian C. M. MacLennan |
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Rok vydání: | 2012 |
Předmět: |
Salmonella
Salmonella Vaccines Immunology B-Lymphocyte Subsets Porins medicine.disease_cause Salmonella typhi Article Microbiology Mice Antigen medicine Animals Immunology and Allergy Typhoid Fever Peritoneal Cavity B cell Mice Knockout Antigens Bacterial biology Polysaccharides Bacterial Salmonella vaccine Antibodies Bacterial Virology Mice Inbred C57BL Vaccination medicine.anatomical_structure Immunization biology.protein Peritoneum Antibody |
Zdroj: | The Journal of Immunology. 189:5527-5532 |
ISSN: | 1550-6606 0022-1767 |
Popis: | Vaccination with purified capsular polysaccharide Vi Ag from Salmonella typhi can protect against typhoid fever, although the mechanism for its efficacy is not clearly established. In this study, we have characterized the B cell response to this vaccine in wild-type and T cell–deficient mice. We show that immunization with typhoid vi polysaccharide vaccine rapidly induces proliferation in B1b peritoneal cells, but not in B1a cells or marginal zone B cells. This induction of B1b proliferation is concomitant with the detection of splenic Vi-specific Ab-secreting cells and protective Ab in Rag1-deficient B1b cell chimeras generated by adoptive transfer-induced specific Ab after Vi immunization. Furthermore, Ab derived from peritoneal B cells is sufficient to confer protection against Salmonella that express Vi Ag. Expression of Vi by Salmonella during infection did not inhibit the development of early Ab responses to non-Vi Ags. Despite this, the protection conferred by immunization of mice with porin proteins from Salmonella, which induce Ab-mediated protection, was reduced postinfection with Vi-expressing Salmonella, although protection was not totally abrogated. This work therefore suggests that, in mice, B1b cells contribute to the protection induced by Vi Ag, and targeting non-Vi Ags as subunit vaccines may offer an attractive strategy to augment current Vi-based vaccine strategies. |
Databáze: | OpenAIRE |
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