Alpha‐momorcharin enhances Nicotiana benthamiana resistance to tobacco mosaic virus infection through modulation of reactive oxygen species

Autor: Feng Zhu, Peng-Xiang Zhu, Fei Xu, Zhao-Lin Ji, Yi-Ming Ma, Yan-Ping Che
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
Antioxidant
medicine.medical_treatment
Transgene
viruses
Ribosome Inactivating Proteins
Soil Science
Nicotiana benthamiana
Gene Expression
Plant Science
Biology
reactive oxygen species (ROS)
01 natural sciences
digestive system
Microbiology
law.invention
resistance
03 medical and health sciences
tobacco mosaic virus (TMV)
law
Tobacco
medicine
Tobacco mosaic virus
Molecular Biology
Gene
Disease Resistance
Plant Diseases
chemistry.chemical_classification
Reactive oxygen species
digestive
oral
and skin physiology
fungi
food and beverages
Original Articles
Antimicrobial
biology.organism_classification
Plants
Genetically Modified
Tobacco Mosaic Virus
030104 developmental biology
chemistry
Recombinant DNA
Original Article
alpha‐momorcharin (α‐MMC)
Reactive Oxygen Species
Agronomy and Crop Science
010606 plant biology & botany
overexpression
Zdroj: Molecular Plant Pathology
ISSN: 1364-3703
1464-6722
Popis: Alpha‐momorcharin (α‐MMC), a member of the plant ribosomal inactivating proteins (RIPs) family, has been proven to exhibit important biological properties in animals, including antiviral, antimicrobial, and antitumour activities. However, the mechanism by which α‐MMC increases plant resistance to viral infections remains unclear. To study the effect of α‐MMC on plant viral defence and how α‐MMC increases plant resistance to viruses, recombinant DNA and transgenic technologies were employed to investigate the role of α‐MMC in Nicotiana benthamiana resistance to tobacco mosaic virus (TMV) infection. Treatment with α‐MMC produced through DNA recombinant technology or overexpression of α‐MMC mediated by transgenic technology alleviated TMV‐induced oxidative damage and reduced the accumulation of reactive oxygen species (ROS) during TMV‐green fluorescent protein infection of N. benthamiana. There was a significant decrease in TMV replication in the upper leaves following local α‐MMC treatment and in α‐MMC‐overexpressing plants relative to control plants. These results suggest that application or overexpression of α‐MMC in N. benthamiana increases resistance to TMV infection. Finally, our results showed that overexpression of α‐MMC up‐regulated the expression of ROS scavenging‐related genes. α‐MMC confers resistance to TMV infection by means of modulating ROS homeostasis through controlling the expression of antioxidant enzyme‐encoding genes. Overall, our study revealed a new crosstalk mechanism between α‐MMC and ROS during resistance to viral infection and provides a framework to understand the molecular mechanisms of α‐MMC in plant defence against viral pathogens.
Application or overexpression of α‐MMC in Nicotiana benthamiana increased resistance to TMV infection by means of modulating ROS homeostasis through controlling the expression of antioxidant enzyme‐encoding genes.
Databáze: OpenAIRE
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