A preliminary study on the proinflammatory mechanisms of Treponema pallidum outer membrane protein Tp92 in human macrophages and HMEC-1 cells

Autor: Tie Zhao, Xi Luo, Wen Liu, Meixia Deng, Xiaohong Zhang, Tiebing Zeng, Feijun Zhao
Rok vydání: 2017
Předmět:
0301 basic medicine
Pyrrolidines
Pyridines
THP-1 Cells
p38 mitogen-activated protein kinases
Interleukin-1beta
030106 microbiology
Microbiology
Amino Acid Chloromethyl Ketones
Cell Line
Proinflammatory cytokine
03 medical and health sciences
chemistry.chemical_compound
Bacterial Proteins
Western blot
Thiocarbamates
Lactate dehydrogenase
NLR Family
Pyrin Domain-Containing 3 Protein
medicine
Humans
Secretion
Treponema pallidum
Cloning
Molecular
Mitogen-Activated Protein Kinase Kinases
Treponema
L-Lactate Dehydrogenase
biology
medicine.diagnostic_test
Interleukin-6
Tumor Necrosis Factor-alpha
Chemistry
Macrophages
Caspase 1
Interleukin-8
Imidazoles
NF-kappa B
Membrane Proteins
biology.organism_classification
Recombinant Proteins
Enzyme assay
030104 developmental biology
Infectious Diseases
Membrane protein
Antigens
Surface
Myeloid Differentiation Factor 88
biology.protein
Cytokines
Signal Transduction
Zdroj: Microbial Pathogenesis. 110:176-183
ISSN: 0882-4010
DOI: 10.1016/j.micpath.2017.06.046
Popis: To determine proinflammatory mechanisms of Treponema pallidum outer membrane protein Tp92 in the early syphilis infection in human macrophages and HMEC-1 cells.Recombinant Tp92 protein was used to stimulate target human macrophages and HMEC-1 cells. PDTC (Pyrrolidinedithiocarbamic acid), SB202190 and Z-YVAD-FMK were used to block the MyD88/NF-κB, MAPKs/p38 and NLRP3/Caspase-1 pathway, respectively. TNF-α, IL-1β, IL-6, IL-8,NLRP3, casepase-1 were detected by ELISA or Western blot. Lactate dehydrogenase (LDH) activity was measured.Tp92 protein could significantly induced the secretion of proinflammatory cytokines TNF-α, IL-1β, IL-6 and IL-8 in HMEC-1 cells, but not in macrophages except IL-8. When MyD88/NF-κB pathway was blocked, differences in the secretion of TNF-α, IL-6 and IL-1β levels and LDH enzyme activity between Tp92 group and Tp92 + PDTC group were not significant (P 0.05) in HMEC-1 cells and macrophages except IL-8(P 0.05). When MAPKs/p38 pathway was blocked, differences in the secretion of TNF-α, IL-1β, IL-6 and IL-8 and LDH enzyme activity both Tp92 group and Tp92 + SB2010190 group were not significant (P 0.05) in HMEC-1 cells and macrophages. In contrast, when NLRP3/Caspase-1 pathway was blocked with Z-YVAD-FMK, TNF-α, IL-6 and IL-1β levels, LDH enzyme activity, and Caspase-1 and NLRP3 protein levels were significantly declined (P 0.05) in HMEC-1 cells except IL-8(P 0.05). The LDH enzyme activity in macrophages was decreased before and after Z-YVAD-FMK blocking (P 0.05),however, differences in the secretion of TNF-α, IL-1β, IL-6 and IL-8 between Tp92 group and Tp92+Z-YVAD-FMK group in macrophages were not significant (P 0.05).Tp92 protein may promote proinflammatory cytokines TNF-α, IL-1β, IL-6 secretion of HMEC-1 cells, but not in macrophages, and increase the LDH enzyme activity of HMEC-1 cells and macrophages through NLRP3/Caspase-1 pathway. However, Tp92 protein may promote IL-8 secretion of HMEC-1 cells and macrophages through MyD88/NF-κB pathway.
Databáze: OpenAIRE
Externí odkaz: