Early plant growth and biochemical responses induced by Azospirillum brasilense Sp245 lipopolysaccharides in wheat (Triticum aestivum L.) seedlings are attenuated by procyanidin B2
| Autor: | Manuel Méndez-Gómez, Ernesto García-Pineda, Elda Castro-Mercado, Juan Vallejo-Ochoa, Laura Nicolasa Suárez-Soria, Mariel López-Marmolejo, Alma Alejandra Hernández-Esquivel |
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| Rok vydání: | 2017 |
| Předmět: |
Lipopolysaccharides
0106 biological sciences 0301 basic medicine Azospirillum brasilense Plant Science Rhizobacteria Plant Roots 01 natural sciences Catechin Microbiology Cell wall 03 medical and health sciences chemistry.chemical_compound B type proanthocyanidin Biflavonoids Proanthocyanidins Biomass Procyanidin B2 Triticum biology Chemistry food and beverages Starch Hydrogen Peroxide Pigments Biological Cell Biology General Medicine biology.organism_classification Plant cell 030104 developmental biology Peroxidases Seedlings Seedling biology.protein lipids (amino acids peptides and proteins) 010606 plant biology & botany Peroxidase |
| Zdroj: | Protoplasma. 255:685-694 |
| ISSN: | 1615-6102 0033-183X |
| DOI: | 10.1007/s00709-017-1180-2 |
| Popis: | This study analyzes the effects of procyanidin B2 on early wheat plant growth and plant biochemical responses promoted by lipopolysaccharides (LPS) derived from the rhizobacteria Azospirillum brasilense Sp245. Measurements of leaf, root length, fresh weight, and dry weight showed in vitro plant growth stimulation 4 days after treatment with A. brasilense as well as LPS. Superoxide anion (O2 ·−) and hydrogen peroxide (H2O2) levels increased in seedling roots treated with LPS (100 μg mL−1). The chlorophyll content in leaf decreased while the starch content increased 24 h after treatment in seedling roots. The LPS treatment induced a high increase in total peroxidase (POX) (EC 1.11.1.7) activity and ionically bound cell wall POX content in roots, when compared to respective controls. Early plant growth and biochemical responses observed in wheat seedlings treated with LPS were inhibited by the addition of procyanidin B2 (5 μg mL−1), a B type proanthocyanidin (PAC), plant-derived polyphenolic compound with binding properties of LPS. All results suggest first that the ionically bound cell wall POX enzymes could be a molecular target of A. brasilense LPS, and second that the recognition or association of LPS by plant cells is required to activate plant responses. This last event could play a critical role during plant growth regulation by A. brasilense LPS. |
| Databáze: | OpenAIRE |
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