Oregano feed supplementation affects glycoconjugates production in swine gut
Autor: | Gabriele Acuti, Federico Maria Tardella, Valerio Faeti, Paola Scocco, Elena De Felice, Cecilia Dall'Aglio, Carolina Pirino, Francesca Mercati |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
Antioxidant
Glycoconjugate medicine.medical_treatment origanum vulgare l medicine.disease_cause swine gut glycoconjugates Origanum vulgare L BAX oxidative stress Article 03 medical and health sciences Intestinal mucosa lcsh:Zoology medicine Secretion Food science lcsh:QL1-991 030304 developmental biology chemistry.chemical_classification 0303 health sciences lcsh:Veterinary medicine General Veterinary Chemistry 0402 animal and dairy science 04 agricultural and veterinary sciences 040201 dairy & animal science Staining medicine.anatomical_structure Duodenum lcsh:SF600-1100 Animal Science and Zoology Immunostaining Oxidative stress |
Zdroj: | Animals : an Open Access Journal from MDPI Animals, Vol 10, Iss 1, p 149 (2020) Animals Volume 10 Issue 1 |
Popis: | This study evaluated the effects of adding oregano aqueous extract (OAE) to the diet of pig slaughtered at finisher stage. Study was performed to identify glycoconjugates and evaluate the oxidative stress levels in the duodenum and colon intestinal tracts. Glycohistochemistry was performed by staining with Periodic acid&ndash Schiff (PAS), Alcian blue (AB) pH 2.5, AB-PAS, AB pH 1, AB pH 0.5, low iron diamine, and high iron diamine. Serial sections were pre-treated with sialidase V before staining with AB pH 2.5 (Sial-AB) preceded or not by saponification. To study oxidative stress, an immunohistochemical analysis was applied to investigate the presence of the oxidative stress target molecule Bcl-2 Associate X protein (BAX). Findings show that oregano aqueous extract supplementation improves the production of the secretion glycoconjugates involved in direct and indirect defense, thus enhancing the protection of the pig intestinal mucosa. Moreover, the reduced BAX protein immunostaining observed in both duodenum and colon of swine of the oregano-supplemented group respect to that observed in the control group suggests an enhanced antioxidant action by oregano adding. Findings could be useful for other studies aiming to reduce antibiotic use and prevent antimicrobial resistance. |
Databáze: | OpenAIRE |
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