Naked PCV-2 cloned genomic DNA is infectious by mucosal (intratracheal or oro-nasal) inoculation
| Autor: | André Jestin, Lionel Bigault, Daniel Dory, Anne-Cécile Hernandez-Nignol, Aurélie Oger, Nicolas Rose, Béatrice Grasland, Roland Cariolet, Maena Le Corvec |
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| Rok vydání: | 2011 |
| Předmět: |
Circovirus
Cancer Research Swine animal diseases Respiratory Mucosa Biology complex mixtures chemistry.chemical_compound Plasmid In vivo Virology Animals Circoviridae Infections Cloning Molecular Cloning Swine Diseases Virulence biology.organism_classification Trachea Porcine circovirus genomic DNA Nasal Mucosa Infectious Diseases Viral replication chemistry Naked DNA DNA Viral DNA |
| Zdroj: | Virus research. 165(1) |
| ISSN: | 1872-7492 |
| Popis: | Porcine circovirus type 2 (PCV-2) is involved in several diseases named porcine circovirus-associated diseases and is transmitted by oro-faecal route. In this study we inoculated porcine-circovirus free piglets by mucosal routes (intratracheal or oro-nasal routes) with a plasmid carrying two copies of PCV-2 genomic DNA and compared the results to the intramuscular route. We observed that this PCV-2 naked DNA serves as template for viral replication and infectious PCV-2 particles are detected in the whole body after parenteral (intramuscular) or mucosal (intratracheal or oro-nasal) delivery. These results suggest that PCV-2 genome could play a role in in vivo transmission. |
| Databáze: | OpenAIRE |
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