The C-Module-Binding Factor Supports Amplification of TRE5-A Retrotransposons in the Dictyostelium discoideum Genome ▿
| Autor: | Oliver Siol, Anika Schmith, Annika Bilzer, Thomas Winckler, Heike Dölz, Alexander Reinhardt |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Genetics
Transcriptional Activation biology Retroelements Transcription Genetic Protein domain Protozoan Proteins Retrotransposon Promoter General Medicine Articles biology.organism_classification Microbiology DNA-binding protein Genome Dictyostelium Dictyostelium discoideum DNA-Binding Proteins Genes Reporter Sense (molecular biology) Promoter Regions Genetic Molecular Biology |
| Popis: | Retrotransposable elements are molecular parasites that have invaded the genomes of virtually all organisms. Although retrotransposons encode essential proteins to mediate their amplification, they also require assistance by host cell-encoded machineries that perform functions such as DNA transcription and repair. The retrotransposon TRE5-A of the social amoeba Dictyostelium discoideum generates a notable amount of both sense and antisense RNAs, which are generated from element-internal promoters, located in the A module and the C module, respectively. We observed that TRE5-A retrotransposons depend on the C-module-binding factor (CbfA) to maintain high steady-state levels of TRE5-A transcripts and that CbfA supports the retrotransposition activity of TRE5-A elements. The carboxy-terminal domain of CbfA was found to be required and sufficient to mediate the accumulation of TRE5-A transcripts, but it did not support productive retrotransposition of TRE5-A. This result suggests different roles for CbfA protein domains in the regulation of TRE5-A retrotransposition frequency in D. discoideum cells. Although CbfA binds to the C module in vitro , the factor regulates neither C-module nor A-module promoter activity in vivo . We speculate that CbfA supports the amplification of TRE5-A retrotransposons by suppressing the expression of an as yet unidentified component of the cellular posttranscriptional gene silencing machinery. |
| Databáze: | OpenAIRE |
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