Improved recovery of human islets from young donor pancreases utilizing increased protease dose to collagenase for digesting peri-islet extracellular matrix
| Autor: | Bakeerathan Gunaratnam, Michael A. Goedde, Praneeth Goli, Venugopal Subhashree, Benjamin Tweed, Sri Prakash Mokshagundam, Gopalakrishnan Loganathan, Appakalai N. Balamurugan, Siddharth Narayanan, Robert C. McCarthy, Michael G. Hughes, Stuart K. Williams, William W. Tucker |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Male endocrine system Tissue and Organ Procurement endocrine system diseases Adolescent medicine.medical_treatment Islets of Langerhans Transplantation 030230 surgery Andrology 03 medical and health sciences Islets of Langerhans Young Adult 0302 clinical medicine In vivo medicine Immunology and Allergy Humans Pharmacology (medical) Collagenases Differential centrifugation Transplantation geography Protease geography.geographical_feature_category business.industry Age Factors Organ Preservation Islet In vitro Tissue Donors Extracellular Matrix medicine.anatomical_structure Collagenase Female Pancreas business medicine.drug Follow-Up Studies Peptide Hydrolases |
| Zdroj: | American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 19(3) |
| ISSN: | 1600-6143 |
| Popis: | Human islet isolation from young donor pancreases (YDP) utilizing the current purified standard dose of collagenase-protease enzyme mixtures often results in the release of a high percentage of mantled islets. Mantled islets are those surrounded by exocrine tissue and are difficult to purify by density gradient centrifugation, leading to poor islet recovery. Based on difference in extracellular matrix, and total collagen content between YDP and old donor pancreas (ODP, > 35 Y) led us to compare results from islet isolation using increased collagenase combination (ICC) or increased protease combination (IPC), to the standard enzyme combination (SEC) in a "trisected" pancreas model to overcome the donor-to-donor variability. These results showed a reduced percentage of mantled islets (17% ± 7.5%) and higher postpurification islet recovery (83.8% ± 5.6%) with IPC. Furthermore, these results were confirmed in 13 consecutive whole pancreas islet isolations utilizing IPC from VitaCyte, Roche, or SERVA collagenase-protease enzyme mixtures. Results obtained from in vitro and in vivo islet functional assessment indicated that islets isolated using IPC retained normal islet morphology, insulin secretion, and the ability to reverse diabetes after transplantation in diabetic nude mice. This is the first report utilizing trisected pancreas to assess the effectiveness of different enzyme combinations to improve islet recovery from young donor pancreases. |
| Databáze: | OpenAIRE |
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