Polymerase chain reaction amplification from Plasmodium falciparum on dried blood spots
| Autor: | Stephen L. Hoffman, George Watt, Lou Fries, Gary W. Long |
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| Rok vydání: | 1995 |
| Předmět: |
Molecular Sequence Data
Plasmodium falciparum Biology Polymerase Chain Reaction Sensitivity and Specificity law.invention law Virology Animals Humans Dried blood Polymerase chain reaction Whole blood DNA Primers Polysaccharide-Lyases Chromatography Spots Filter paper Base Sequence Vaccine trial DNA Protozoan biology.organism_classification Acridine Orange Blood film Infectious Diseases Heparin Lyase Parasitology |
| Zdroj: | The American journal of tropical medicine and hygiene. 52(4) |
| ISSN: | 0002-9637 |
| Popis: | We report a simple method for the polymerase chain reaction (PCR) amplification of whole blood samples collected on filter paper. The blood spot was used directly in the PCR after treatment with methanol. We evaluated this assay using clinical samples collected from subjects in a Plasmodium falciparum vaccine trial and from samples collected during a hospital-based study in Thailand. Specimens prepared from heparinized blood samples were successfully amplified following pretreatment with heparinase. Sensitivity was 100% when compared with thick blood film results in the vaccine trial (range = 4-60 parasites/microliters, median = 8/microliters) and 94.6% (range = 3-133,988 parasites/microliters, median = 616/microliters) in the hospital study. |
| Databáze: | OpenAIRE |
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