E-cigarette constituents propylene glycol and vegetable glycerin decrease glucose uptake and its metabolism in airway epithelial cells in vitro
| Autor: | Victoria Schroeder, Maximillian Woodall, J. Jacob, James P. Garnett, Robert Tarran, I. Khan, Deborah L. Baines, B. Kenyon, Kameljit K. Kalsi, E. Davis |
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| Rok vydání: | 2020 |
| Předmět: |
Glycerol
0301 basic medicine Pulmonary and Respiratory Medicine glycerin Physiology Glucose uptake Respiratory System Electronic Nicotine Delivery Systems 03 medical and health sciences 0302 clinical medicine Physiology (medical) medicine Membrane fluidity Humans Glycolysis biology Chemistry Glucose transporter Biological Transport Epithelial Cells Cell Biology Metabolism Propylene Glycol Molecular biology Epithelium Glucose 030104 developmental biology medicine.anatomical_structure 030228 respiratory system airway electronic cigarettes biology.protein GLUT1 Mannitol Research Article medicine.drug |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology |
| ISSN: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.00123.2020 |
| Popis: | Electronic nicotine delivery systems, or e-cigarettes, utilize a liquid solution that normally contains propylene glycol (PG) and vegetable glycerin (VG) to generate vapor and act as a carrier for nicotine and flavorings. Evidence indicated these “carriers” reduced growth and survival of epithelial cells including those of the airway. We hypothesized that 3% PG or PG mixed with VG (3% PG/VG, 55:45) inhibited glucose uptake in human airway epithelial cells as a first step to reducing airway cell survival. Exposure of H441 or human bronchiolar epithelial cells (HBECs) to PG and PG/VG (30–60 min) inhibited glucose uptake and mitochondrial ATP synthesis. PG/VG inhibited glycolysis. PG/VG and mannitol reduced cell volume and height of air-liquid interface cultures. Mannitol, but not PG/VG, increased phosphorylation of p38 MAPK. PG/VG reduced transepithelial electrical resistance, which was associated with increased transepithelial solute permeability. PG/VG decreased fluorescence recovery after photobleaching of green fluorescent protein-linked glucose transporters GLUT1 and GLUT10, indicating that glucose transport function was compromised. Puffing PG/VG vapor onto the apical surface of primary HBECs for 10 min to mimic the effect of e-cigarette smoking also reduced glucose transport. In conclusion, short-term exposure to PG/VG, key components of e-cigarettes, decreased glucose transport and metabolism in airway cells. We propose that this was a result of PG/VG reduced cell volume and membrane fluidity, with further consequences on epithelial barrier function. Taking these results together, we suggest these factors contribute to reduced defensive properties of the epithelium. We propose that repeated/chronic exposure to these agents are likely to contribute to airway damage in e-cigarette users. |
| Databáze: | OpenAIRE |
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