The combined effect of magnetic and electric fields using on/off infrared light on the blood sugar level and the diameter of Langerhans islets of diabetic mice
| Autor: | S. Suhariningsih, Suryani Dyah Astuti, Saikhu Akhmad Husen, Dwi Winarni, Dian Astri Rahmawati, Akhmad Taufiq Mukti, Alfian Pramudita Putra, Muhammad Miftahussurur |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Veterinary medicine medicine.medical_treatment Intraperitoneal injection infrared light langerhans islets diameter H&E stain Blood sugar magnetic field diabetic mice SF1-1100 blood sugar level 03 medical and health sciences 0302 clinical medicine Diabetes mellitus Internal medicine SF600-1100 medicine 030304 developmental biology 0303 health sciences geography geography.geographical_feature_category General Veterinary Chemistry Insulin medicine.disease Streptozotocin Islet electric field Animal culture medicine.anatomical_structure Endocrinology 030220 oncology & carcinogenesis Pancreas Research Article medicine.drug |
| Zdroj: | Veterinary World, Vol 13, Iss 10, Pp 2286-2293 (2020) Veterinary World |
| ISSN: | 2231-0916 0972-8988 |
| DOI: | 10.14202/vetworld.2020.2286-2293 |
| Popis: | Background and Aim: At present, diabetes is treated with oral antidiabetic medicines, such as sulfonylureas and thiazolidine, as well as insulin injection. However, these methods have several shortcomings. Therefore, alternatives for treating diabetes mellitus (DM) are needed. This study aims to determine the combined effect of magnetic and electric fields on blood sugar levels and the diameter of Langerhans islets of diabetic mice. Materials and Methods: Induction of DM in mice was carried out by administering lard for 2 weeks and continued with an intraperitoneal injection of streptozotocin, dissolved in a 4.5 pH citrate buffer, and administered in a dose of 30 mg/kg bodyweight for 5 days. Treatments were used in combination with magnetic and electric fields using on/off infrared light. Blood samples were pipetted through the tip of mice's tails to establish the blood sugar level for each individual mouse. Histology preparation of the pancreas organ was affected using the histology standard as well as hematoxylin and eosin staining methods. Langerhans islet diameter data were analyzed using analysis of variance followed by Duncan's multiple range test. Data analysis was performed at α=0.05. Results: The results showed that the combined treatment of permanent magnetic and unidirectional electric fields (PS) caused changes in blood sugar levels that were not significantly different from the normal control group. The PS treatment improved the diameter of the Langerhans islets but not to a significant degree compared to other treatments. Conclusion: The use of PS treatment is effective for reducing the blood sugar levels of diabetic mice and improving the diameter of their Langerhans islets. |
| Databáze: | OpenAIRE |
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