Evidence for the Contribution of Surface Potential to the Transtonoplast Potential Difference Measured on Isolated Vacuoles with Microelectrodes
| Autor: | Rémy Gibrat, Jean-Pierre Grouzis, Claude Grignon, H. Barbier-Brygoo, J. Guern, Charles Romieu |
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| Přispěvatelé: | Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre National de la Recherche Scientifique (CNRS), Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) |
| Jazyk: | angličtina |
| Rok vydání: | 1984 |
| Předmět: |
0106 biological sciences
Membrane potential 0303 health sciences biology Chemistry [SDV]Life Sciences [q-bio] General Medicine Vacuole Acer pseudoplatanus biology.organism_classification 01 natural sciences Transmembrane protein 03 medical and health sciences Electrophoresis Biochemistry Pulmonary surfactant Zeta potential [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Electric potential ComputingMilieux_MISCELLANEOUS 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Zeitschrift für Pflanzenphysiologie Zeitschrift für Pflanzenphysiologie, 1984, 114 (3), pp.215-219. ⟨10.1016/S0044-328X(84)80013-6⟩ |
| ISSN: | 0044-328X |
| DOI: | 10.1016/S0044-328X(84)80013-6⟩ |
| Popis: | Summary In order to elucidate the origin of the transtonoplast potential difference (PD) measured on isolated vacuoles, the possible contribution of the surface potential to the transmembrane PD was studied. The PD and the electrophoretic mobility were measured on vacuolar preparations, isolated from Beta vulgaris roots and Acer pseudoplatanus cells. Zeta potential was calculated from the electrophoretic mobility. The changes in zeta potential, in response to pH or to a cationic surfactant, were shown to induce parallel variations in the PD. These results suggest that the surface potential could contribute to the transmembrane PD of isolated vacuoles. Various hypotheses on the origin of this transtonoplast PD are discussed. |
| Databáze: | OpenAIRE |
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