F-box only protein 9 is required for adipocyte differentiation
| Autor: | Kyeong Won Lee, Hye Seung Jung, Young Min Cho, Young Joo Park, Sung Soo Chung, Soo Heon Kwak, Kyong Soo Park, Byung Yong Ahn, Hak Mo Lee |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Small interfering RNA Biophysics Biochemistry Mice chemistry.chemical_compound Ubiquitin Internal medicine Adipocyte Enhancer binding Adipocytes medicine Animals Obesity Molecular Biology Gene knockdown Adipogenesis biology F-Box Proteins Cell Differentiation Cell Biology Transfection Ubiquitin ligase Cell biology Mice Inbred C57BL Endocrinology chemistry biology.protein |
| Zdroj: | Biochemical and Biophysical Research Communications. 435:239-243 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2013.04.072 |
| Popis: | The objective of this study is to investigate whether F-box only protein 9 (FBXO9), an ubiquitination E3 ligase, has a functional role in adipocyte differentiation. Expression of FBXO9 was compared between obese mice and control lean mice using real-time PCR. Also, expression pattern of FBXO9 was monitored during 3T3-L1 adipocyte differentiation. FBXO9 was highly expressed in obese mice, and increased in the early stages of adipogenesis. To verify a functional role of FBXO9 in adipogenesis, FBXO9 was knocked down using transfection of siRNAs against FBXO9 into 3T3-L1 cells during the induction of adipogenesis. Knockdown of FBXO9 in early stage of adipogenesis almost completely inhibited adipogenesis, and CCAAT/enhancer binding protein β (C/EBPβ) levels were significantly reduced. However, the cells stably expressing C/EBPβ were fairly differentiated into adipocytes in the FBXO9 knockdown condition. These results suggest that FBXO9 is required for adipocyte differentiation, and C/EBPβ plays a role in the effect of FBXO9 on adipogenesis. |
| Databáze: | OpenAIRE |
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