Protective immune response in mice induced by a suicidal DNA vaccine encoding NTPase-II gene of Toxoplasma gondii
| Autor: | Lina Zheng, Yujing Wan, Feng Tan, Qianqian Hua, Yue Hu, Chang-Wang Pan, Jiaxin Lin, Shoufeng Ren, Fangjun Luo, Xiangzhi Li, Guizhen Xie |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Protozoan Vaccines Veterinary (miscellaneous) 030231 tropical medicine Protozoan Proteins Injections Intramuscular DNA vaccination 03 medical and health sciences Mice 0302 clinical medicine Immune system Vaccines DNA Animals Replicon Immunity Cellular Mice Inbred BALB C biology Electroporation Antibody titer Toxoplasma gondii Transfection biology.organism_classification Nucleoside-Triphosphatase Virology 030104 developmental biology Infectious Diseases Toxoplasmosis Animal Insect Science Immunoglobulin G Immunology Cytokines Parasitology Toxoplasma CD8 |
| Zdroj: | Acta tropica. 166 |
| ISSN: | 1873-6254 |
| Popis: | DNA-based alphaviral RNA replicon vectors, also called suicidal DNA vectors, have been employed to alleviate biosafety concerns attribution to its ability to induce apoptotic cell death of the transfected cells. Toxoplasma gondii nucleoside triphosphate hydrolase-II (TgNTPase-II), which facilitates the parasite to salvage purines from the host cell for survival and replication, have been demonstrated to be a potential vaccine candidate for toxoplasmosis. Herein, we evaluated the immunogenic potential of a suicidal DNA vaccine encoding TgNTPase-II gene, pDREP-TgNTPase-II, delivered intramuscularly in combination with electroporation. Immunization of mice with pDREP-TgNTPase-II elicited specific humoral responses, with high IgG antibody titers and a mixed IgG1/IgG2a response. The cellular immune response was associated with high level production of IFN-γ, IL-2, IL-10 cytokines and low level IL-4 production as well as the increase of the percentage of CD8+ T cells, indicating that a Th1 predominant response was elicited. Furthermore, mice vaccinated with this suicidal DNA vaccine displayed partial protection against acute infection with the virulent RH strain as well as chronic infection with PRU cyst, which shows 77.7% and 71.4% reduction in brain cyst burden in comparison to PBS and pDREP-eGFP control group, respectively. Based on the cellular and antibody responses, the suicidal DNA vaccine elicited a Th1-predominant immune response against T. gondii challenge. |
| Databáze: | OpenAIRE |
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