Distinct effect of age, sex, and CMV seropositivity on dendritic cells and monocytes in human blood

Autor: Antoine Blancher, Sandrine Sourdet, Nicolas Congy-Jolivet, Kevin Guedj, Francis Roubinet, Bénédicte Puissant-Lubrano, Fati Nourhashemi, Sophie Guyonnet, Pol André Apoil
Přispěvatelé: CHU Toulouse [Toulouse], Laboratoire d'Immunogénétique Moléculaire (LIMT), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Unité différenciation épidermique et auto-immunité rhumatoïde (UDEAR), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Etablissement français du sang [Pyrénées-Méditerranée] (EFS Pyrénées-Méditerranée), Centre de Physiopathologie de Toulouse-Purpan (INSERM U563 - CNRS UMR1037), Centre National de la Recherche Scientifique (CNRS)-Centre de lutte contre le cancer (CLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse]-CHU Toulouse [Toulouse]-Institut Claudius Regaud
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
Aging
Myeloid
Cytomegalovirus
Cell Count
MESH: Flow Cytometry
Cell Separation
Antibodies
Viral
Monocytes
MESH: Aged
80 and over
0302 clinical medicine
Immunophenotyping
MESH: Sex
Immunology and Allergy
MESH: Dendritic Cells / immunology
MESH: Antibodies
Viral / blood
Whole blood
Aged
80 and over
MESH: Aged
CD64
MESH: Middle Aged
MESH: Monocytes / immunology
MESH: Cytomegalovirus / immunology
MESH: Blood Cells / immunology
Middle Aged
Flow Cytometry
medicine.anatomical_structure
MESH: Young Adult
Cytomegalovirus Infections
[SDV.IMM]Life Sciences [q-bio]/Immunology
Female
Sex
Blood drawing
Adult
MESH: Immunophenotyping
Immunology
Biology
MESH: Cell Separation
Young Adult
03 medical and health sciences
medicine
Humans
MESH: Cytomegalovirus Infections / immunology
Aged
MESH: Aging / immunology
Blood Cells
MESH: Humans
MESH: Cell Count
Monocyte
MESH: Adult
Dendritic Cells
Cell Biology
Dendritic cell
MESH: Male
030104 developmental biology
MESH: Female
CD163
030215 immunology
Zdroj: Immunology and Cell Biology
Immunology and Cell Biology, Nature Publishing Group, 2018, 96 (1), pp.114-120. ⟨10.1111/imcb.1004⟩
ISSN: 0818-9641
1440-1711
DOI: 10.1111/imcb.1004
Popis: International audience; We analyzed the impact of age, sex, and CMV on blood monocyte and dendritic cell (DC) subpopulations in 256 healthy individuals aged from 19 to 96 years. Flow cytometry was performed on whole blood within the 4 h following blood drawing. Myeloid (mDC) and plasmacytoid DC (pDC), classical, intermediate, and nonclassical monocytes were enumerated by means of TruCount tubes (BD Biosciences). We provided reference values for mDC, pDC and the three monocyte subpopulations. The numbers of classical, intermediate, and nonclassical monocytes slightly increased with age while the numbers of mDC and pDC did not vary significantly. The level of expression of CD64 and CD163 on monocytes significantly increased with age while HLA-DR expression did not vary significantly. More precisely, CD163 expression level on intermediate monocyte slightly increased with age in women only (Spearman P = 0.019) while CD64 expression increased on monocytes in CMV-positive individuals only. We observed that sex had almost no impact on the numbers of monocytes and DC and on their expression level of CD64 and HLA-DR. We observed a significant decrease in the numbers of pDC with age in CMV-positive individuals, but not in CMV negative individuals. This suggests that the lifelong subclinical infection by CMV could influence the number of circulating DC of lymphoid origin. In contrast, CMV serostatus had no significant impact on absolute numbers of mDC and monocytes.
Databáze: OpenAIRE
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