GRP78 as a marker of pre-eclampsia: an exploratory study

Autor: Olivier Irion, Ruth Landau, Marie-Benoîte Cohen, Paul Bischof, Igor Charvet, M. Morales, A. Laverriere
Rok vydání: 2009
Předmět:
Embryology
medicine.medical_specialty
Uterus
Biological Markers/analysis/*metabolism
Enzyme-Linked Immunosorbent Assay
Gestational Age
Biology
Preeclampsia
Andrology
Pre-Eclampsia
Pregnancy
Pre-Eclampsia/*diagnosis/*metabolism
Internal medicine
Genetics
medicine
Humans
Heat-Shock Proteins/analysis/immunology/*metabolism
Molecular Biology
Endoplasmic Reticulum Chaperone BiP
reproductive and urinary physiology
Heat-Shock Proteins
Autoantibodies
ddc:618
Eclampsia
Predictive marker
ddc:617
Autoantibody
Obstetrics and Gynecology
Gestational age
Trophoblast
Cell Biology
medicine.disease
Flow Cytometry
Immunohistochemistry
Pregnancy Trimester
First
Endocrinology
medicine.anatomical_structure
Reproductive Medicine
embryonic structures
Female
Autoantibodies/immunology
Biomarkers
Developmental Biology
Zdroj: Molecular Human Reproduction, Vol. 15, No 9 (2009) pp. 569-574
ISSN: 1460-2407
1360-9947
Popis: Although the exact mechanisms that lead to shallow invasion or defective trophoblastic differentiation in pre-eclampsia are still unknown, it is widely admitted that the etiology of pre-eclampsia is a defect in trophoblast invasion of the uterine spiral arteries. We have previously observed that the status of a chaperone protein, glucose regulated protein 78 (GRP78) is associated with the invasive properties of cytotrophoblastic cells; we therefore hypothesized that circulating GRP78 could serve as a diagnostic tool in pre-eclampsia. In a prospective case-control study, we quantified GRP78 autoantibodies, complexes of GRP78 with autoantibodies and GRP78 (C-term fragment, N-term fragment and full-length GRP78) by ELISA. Plasma from women diagnosed with pre-eclampsia (n = 16), from women during the first trimester of pregnancy who subsequently developed pre-eclampsia (n = 10) and from healthy pregnant women (controls, n = 58 at term, n = 26 at first trimester) were analysed and compared. We observed no significant difference between pre-eclamptic and healthy pregnant women for autoantibodies-GRP78 complexes or total GRP78 at both first trimester and at delivery. In contrast, the ratio of C-terminal GRP78 over full length GRP78 was significantly different in plasma of pre-eclamptic patients as compared with controls both during first trimester (P < 0.004) and at term (P < 0.0001). Our findings suggest that circulating C-terminal GRP78 reflect the invasive properties of cells, and could be used as a predictive marker for pre-eclampsia early in pregnancy.
Databáze: OpenAIRE
Externí odkaz: