Multi-residue determination of antibiotics in fish by liquid chromatography-tandem mass spectrometry
| Autor: | Tomasz Bladek, Kamila Mitrowska, Andrzej Posyniak, M. Gbylik, Jan Zmudzki |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
medicine.drug_class
Health Toxicology and Mutagenesis Antibiotics Toxicology Tandem mass spectrometry chemistry.chemical_compound Limit of Detection Tandem Mass Spectrometry Liquid chromatography–mass spectrometry medicine Animals Detection limit Reproducibility Chromatography Chemistry Extraction (chemistry) Fishes Public Health Environmental and Occupational Health Reproducibility of Results Heptafluorobutyric acid General Chemistry General Medicine Repeatability Drug Residues Anti-Bacterial Agents Food Science |
| Zdroj: | Food Additives & Contaminants: Part A. 30:940-948 |
| ISSN: | 1944-0057 1944-0049 |
| Popis: | A multi-residue method for the determination of 34 antibacterial drugs (three aminoglycosides, nine β-lactams, nine fluoroquinolones, three macrolides, five sulfonamides, trimethoprim and four tetracyclines) in fish samples by LC-MS/MS was developed. The procedure enables the isolation of residues based on double extraction of the fish sample with m-phosphoric acid and heptafluorobutyric acid as an ion-pair agent and acetonitrile. All compounds were determined at the same time on a C18 column with gradient elution. The method was validated according to the requirements of European Decision 2002/657/EC. Specificity, recovery, repeatability, within-laboratory reproducibility, decision limit CCα and detection capability CCβ were calculated. CCα ranged from 55.3 to 1085 µg kg(-1) and CCβ ranged from 59.5 to 1141 µg kg(-1). The overall recoveries were from 96% to 111% with respect to the internal standards. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |