New Caspases' inhibitors belonging to the serpin superfamily : a novel key control point of apoptosis in mammalian tissues
| Autor: | Mohammed Gagaoua, Abdelghani Boudjellal, Ahmed Ouali, Brigitte Picard, Samira Becila, Miguel Angel Sentandreu, Yasmine Boudida |
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| Přispěvatelé: | INATAA, Université Mentouri Constantine [Algérie] (UMC), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
Proteases myoblast differentiation [SDV.BIO]Life Sciences [q-bio]/Biotechnology bovine apoptosis caspases serpins Cell Regulator Biotechnologies 030204 cardiovascular system & hematology 03 medical and health sciences 0302 clinical medicine Biologie animale medicine Myocyte Caspase ComputingMilieux_MISCELLANEOUS 030304 developmental biology Animal biology 0303 health sciences biology Myogenesis [SDV.BA]Life Sciences [q-bio]/Animal biology Intrinsic apoptosis General Medicine Agricultural sciences Cell biology medicine.anatomical_structure Apoptosis biology.protein Sciences agricoles |
| Zdroj: | Advances in Bioscience and Biotechnology Advances in Bioscience and Biotechnology, 2012, 3 (6), pp.740-750. ⟨10.4236/abb.2012.326095⟩ Advances in Bioscience and Biotechnology 6 (3), 740-750. (2012) |
| Popis: | The present report overviews a new family of bovine serpins able to inhibit pseudo-irreversibly initiator and effector caspases, a group of cysteine proteases in charge of cell dismantling during apoptosis, a finely regulated cell death process. The 8 members identified at the gene level showed a high homology with human SERPINA3 and were therefore designed bovSERPINA3-1 to A3-8. At least six of them are able to inhibit caspases. Two of them (bovSERPINA3-1 and A3-3) have been purified from bovine muscle and extensively investigated during these last years. After a general presentation of the serpin superfamily, the kinetic aspects of their interaction with human cas-pases 3 and 8 were studied and findings obtained suggest that caspases could be their target enzymes in living cells. In muscle and primary myoblast in culture, they showed an intracellular localization and because of their high level in blood, they can be exported. Two biological functions (potential regulator of apoptosis and expression during myoblast differentiation) were investigated and it was concluded that they are very likely a efficient regulator of apoptosis, a proposal supported by their high expression in proliferating myoblast (cell survival is essential during this differentiation phase) but not in myotubes. |
| Databáze: | OpenAIRE |
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