Patterns of expression and secretion of vascular endothelial growth factor in malignant soft-tissue tumours
| Autor: | Marcus Lehnhardt, Edina Tolnay, Cornelius Kuhnen, Thomas Muehlberger, Klaus Michael Müller, Peter M. Vogt |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Vascular Endothelial Growth Factor A Cancer Research medicine.medical_specialty Pathology Adolescent Angiogenesis medicine.medical_treatment Enzyme-Linked Immunosorbent Assay Endothelial Growth Factors In situ hybridization Biology chemistry.chemical_compound Internal medicine medicine Humans In Situ Hybridization Aged Aged 80 and over Lymphokines Hematology Vascular Endothelial Growth Factors Sarcoma General Medicine Middle Aged medicine.disease Immunohistochemistry Vascular endothelial growth factor Vascular endothelial growth factor A Cytokine Oncology chemistry Female |
| Zdroj: | Journal of Cancer Research and Clinical Oncology. 126:219-225 |
| ISSN: | 1432-1335 0171-5216 |
| DOI: | 10.1007/s004320050036 |
| Popis: | Vascular endothelial growth factor (VEGF) is an important cytokine especially in the process of tumour angiogenesis. A total of 46 soft-tissue sarcomas were analysed for the expression and possible secretion of VEGF by immunohistochemistry, in-situ hybridisation, and enzyme-linked immunosorbent assays (ELISA). VEGF was demonstrated immunohistochemically in tumour tissue in 45 of 46 cases. The detection of mRNA transcripts yielded evidence of synthesis of VEGF in these sarcomas. ELISA could be performed in 21 cases. Higher concentrations of VEGF were found in tumour-related intraoperatively sampled venous blood in 16 out of 21 patients (76%) than in systemic concentrations taken preoperatively. The results indicated the secretion of VEGF by tumour cells although these raised concentrations were not statistically significant. In 12 out of these 16 patients (75%) a concurrent moderate to strong immunoexpression of VEGF was detected. The relevance of VEGF blood concentrations as a potential "progress parameter" for the course of disease remains questionable. This is mainly due to the lack of statistical significance in the difference between systemic VEGF concentrations in patients and those of a control group. Further long-term follow-up studies are needed, which should include patients with tumour recurrences. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink