Categorisation of sugar acid dehydratases in Aspergillus niger
Autor: | Merja Penttilä, Peter Richard, Joosu Kuivanen, Hanna Keränen, Satu Hilditch, Francine Aline Motter |
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Jazyk: | angličtina |
Rok vydání: | 2014 |
Předmět: |
D-Galactonate
Saccharomyces cerevisiae Genes Fungal Microbiology Sugar acids Fungal Proteins chemistry.chemical_compound Open Reading Frames L-Galactonate Genetics Dehydratase Sugar Gene Hydro-Lyases chemistry.chemical_classification biology L-Rhamnonate Aspergillus niger 2-Keto-3-deoxy acid Sugar Acids biology.organism_classification Open reading frame L-Fuconate Biochemistry chemistry D-Galacturonic acid |
Zdroj: | Motter, F A, Kuivanen, J, Keränen, H, Hilditch, S, Penttilä, M & Richard, P 2014, ' Categorisation of sugar acid dehydratases in Aspergillus niger ', Fungal Genetics and Biology, vol. 64, pp. 67-72 . https://doi.org/10.1016/j.fgb.2013.12.006 |
ISSN: | 1096-0937 1087-1845 |
Popis: | In the genome of Aspergillus niger five genes were identified coding for proteins with homologies to sugar acid dehydratases. The open reading frames were expressed in Saccharomyces cerevisiae and the activities tested with a library of sugar acids. Four genes were identified to code for proteins with activities with sugar acids: an l-galactonate dehydratase (gaaB), two d-galactonate dehydratases (dgdA, dgdB) and an l-rhamnonate dehydratase (lraC). The specificities of the proteins were characterised. The l-galactonate dehydratase had highest activity with l-fuconate, however it is unclear whether the enzyme is involved in l-fuconate catabolism. None of the proteins showed activity with galactaric acid or galactarolactone. |
Databáze: | OpenAIRE |
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