Two simplified fluorescent staining techniques to observe infection structures of the oomycete Plasmopara viticola in grapevine leaf tissues
| Autor: | Didier Merdinoglu, Ana María Díez-Navajas, Anne Poutaraud, Charles Greif |
|---|---|
| Přispěvatelé: | Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I |
| Rok vydání: | 2007 |
| Předmět: |
0106 biological sciences
PLASMOPARA VITICOLA Potassium Compounds Zoospore [SDV]Life Sciences [q-bio] General Physics and Astronomy ANILINE BLUE 01 natural sciences Microbiology 03 medical and health sciences Pathosystem Structural Biology OOMYCETES Haustorium MYCELIUM Hydroxides HAUSTORIA Vitis EPIFLUORESCENCE General Materials Science Fluorescent Dyes Plant Diseases 030304 developmental biology Oomycete 0303 health sciences Aniline Compounds Staining and Labeling biology Sporangium Cell Biology Spores Fungal biology.organism_classification Staining Plant Leaves Microscopy Fluorescence Plasmopara viticola BLANKOPHOR Downy mildew 010606 plant biology & botany |
| Zdroj: | Micron Micron, Elsevier, 2007, 38 (6), pp.680-683. ⟨10.1016/j.micron.2006.09.009⟩ |
| ISSN: | 0968-4328 |
| DOI: | 10.1016/j.micron.2006.09.009 |
| Popis: | International audience; Plasmopara viticola, the causal agent of grapevine downy mildew, is an obligate biotrophic oomycete that grows in the intercellular spaces of host tissues and develops haustoria in the cells. Histological observations are the most effective methods to visualize and quantify the development of the infection structures. We chose two staining techniques leading to high resolution and contrast between parasite structures and host-plant tissues with a minimum of sample preparation: Blankophor and KOH-aniline blue fluorescent stainings. Blankophor (50 ppm in water or 15% KOH) staining was used to study the zoospore encystement on the leaf surface after release from sporangia. The aniline blue dye (0.05% in 0.067 M K2HPO4, pH 9-9.5, after hot KOH whitening) was used to observe the invasive structures inside host tissues that lead to the production of sporangiophores and infectious sporangia. We tested modifications of some parameters of the procedures to determine the most appropriate for high throughput analyses adapted to our pathosystem and equipment facilities. |
| Databáze: | OpenAIRE |
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