| Autor: |
Abdalla M. Abdrabou, Bill T. V. Duong, Kangfu Chen, Randy Singh Atwal, Mahmoud Labib, Sichun Lin, Stephane Angers, Shana O. Kelley |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
ACS central science. 8(12) |
| ISSN: |
2374-7943 |
| Popis: |
Genome-wide loss-of-function screens are critical tools to identify novel genetic regulators of intracellular proteins. However, studying the changes in the organelle-specific expression profile of intracellular proteins can be challenging due to protein localization differences across the whole cell, hindering context-dependent protein expression and activity analyses. Here, we describe nuPRISM, a microfluidics chip specifically designed for large-scale isolated nuclei sorting. The new device enables rapid genome-wide loss-of-function phenotypic CRISPR-Cas9 screens directed at intranuclear targets. We deployed this technology to identify novel genetic regulators of β-catenin nuclear accumulation, a phenotypic hallmark of |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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