Structure and Sequence of the Sex Determining Locus in Two Wild Populations of Nile Tilapia

Autor: Helena D'Cotta, Etienne Bezault, Jean-François Baroiller, David J. Penman, Cécile Triay, Matthew A. Conte, Thomas D. Kocher, Frances E. Clark
Přispěvatelé: Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), University of Maryland [College Park], University of Maryland System, Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), University of Stirling, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
Male
Genetic Linkage
01 natural sciences
Nile tilapia
Genetics (clinical)
Genetics
Oreochromis niloticus
Chromosome Mapping
male duplication
Cichlids
genetic structures [EN]
génétique animale
amh
Female
Genetic Markers
Sexage
lcsh:QH426-470
Locus
Quantitative Trait Loci
Locus (genetics)
Biology
Y chromosome
010603 evolutionary biology
Polymorphism
Single Nucleotide

DNA sequencing
Article
03 medical and health sciences
sex-determinant
Sex-determination system
Animals
Domestication
Gene
Y-chromosome
B chromosome
[SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE]
Détermination du sexe
Whole Genome Sequencing
Sequence Analysis
DNA

Sex Determination Processes
L10 - Génétique et amélioration des animaux
biology.organism_classification
[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics
lcsh:Genetics
030104 developmental biology
Zdroj: Genes, Vol 11, Iss 1017, p 1017 (2020)
Genes
Genes, MDPI, 2020, 11 (9), pp.1017. ⟨10.3390/genes11091017⟩
Volume 11
Issue 9
Genes, 2020, 11 (9), pp.1017. ⟨10.3390/genes11091017⟩
ISSN: 2073-4425
DOI: 10.3390/genes11091017⟩
Popis: In domesticated strains of the Nile tilapia, phenotypic sex has been linked to genetic variants on linkage groups 1, 20 and 23. This diversity of sex-loci might reflect a naturally polymorphic sex determination system in Nile tilapia, or it might be an artefact arising from the process of domestication. Here, we searched for sex-determiners in wild populations from Kpandu, Lake Volta (Ghana-West Africa), and from Lake Koka (Ethiopia-East Africa) that have not been subjected to any genetic manipulation. We analysed lab-reared families using double-digest Restriction Associated DNA sequencing (ddRAD) and analysed wild-caught males and females with pooled whole-genome sequencing (WGS). Strong sex-linked signals were found on LG23 in both populations, and sex-linked signals with LG3 were observed in Kpandu samples. WGS uncovered blocks of high sequence coverage, suggesting the presence of B chromosomes. We confirmed the existence of a tandem amh duplication in LG23 in both populations and determined its breakpoints between the oaz1 and dot1l genes. We found two common deletions of ~5 kb in males and confirmed the presence of both amhY and amh∆Y genes. Males from Lake Koka lack both the previously reported 234 bp deletion and the 5 bp frameshift-insertion that creates a premature stop codon in amh∆Y.
Databáze: OpenAIRE