Structure and Sequence of the Sex Determining Locus in Two Wild Populations of Nile Tilapia
Autor: | Helena D'Cotta, Etienne Bezault, Jean-François Baroiller, David J. Penman, Cécile Triay, Matthew A. Conte, Thomas D. Kocher, Frances E. Clark |
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Přispěvatelé: | Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), University of Maryland [College Park], University of Maryland System, Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), University of Stirling, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE) |
Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine Male Genetic Linkage 01 natural sciences Nile tilapia Genetics (clinical) Genetics Oreochromis niloticus Chromosome Mapping male duplication Cichlids genetic structures [EN] génétique animale amh Female Genetic Markers Sexage lcsh:QH426-470 Locus Quantitative Trait Loci Locus (genetics) Biology Y chromosome 010603 evolutionary biology Polymorphism Single Nucleotide DNA sequencing Article 03 medical and health sciences sex-determinant Sex-determination system Animals Domestication Gene Y-chromosome B chromosome [SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE] Détermination du sexe Whole Genome Sequencing Sequence Analysis DNA Sex Determination Processes L10 - Génétique et amélioration des animaux biology.organism_classification [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics lcsh:Genetics 030104 developmental biology |
Zdroj: | Genes, Vol 11, Iss 1017, p 1017 (2020) Genes Genes, MDPI, 2020, 11 (9), pp.1017. ⟨10.3390/genes11091017⟩ Volume 11 Issue 9 Genes, 2020, 11 (9), pp.1017. ⟨10.3390/genes11091017⟩ |
ISSN: | 2073-4425 |
DOI: | 10.3390/genes11091017⟩ |
Popis: | In domesticated strains of the Nile tilapia, phenotypic sex has been linked to genetic variants on linkage groups 1, 20 and 23. This diversity of sex-loci might reflect a naturally polymorphic sex determination system in Nile tilapia, or it might be an artefact arising from the process of domestication. Here, we searched for sex-determiners in wild populations from Kpandu, Lake Volta (Ghana-West Africa), and from Lake Koka (Ethiopia-East Africa) that have not been subjected to any genetic manipulation. We analysed lab-reared families using double-digest Restriction Associated DNA sequencing (ddRAD) and analysed wild-caught males and females with pooled whole-genome sequencing (WGS). Strong sex-linked signals were found on LG23 in both populations, and sex-linked signals with LG3 were observed in Kpandu samples. WGS uncovered blocks of high sequence coverage, suggesting the presence of B chromosomes. We confirmed the existence of a tandem amh duplication in LG23 in both populations and determined its breakpoints between the oaz1 and dot1l genes. We found two common deletions of ~5 kb in males and confirmed the presence of both amhY and amh∆Y genes. Males from Lake Koka lack both the previously reported 234 bp deletion and the 5 bp frameshift-insertion that creates a premature stop codon in amh∆Y. |
Databáze: | OpenAIRE |
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