Anti-Her-2/neu antibody induces apoptosis in Her-2/neu overexpressing breast cancer cells independently from p53 status
| Autor: | T. Brodowicz, Margarethe Rudas, Sandra Tomek, W. Koestler, C.C. Zielinski, Christoph Wiltschke, C. Ludwig, M. Hejna, Daniela Kandioler, Alexandra C. Budinsky, R Kunstfeld |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
p53
Cancer Research Programmed cell death Time Factors Genotype Tumor suppressor gene Receptor ErbB-2 HER-2/neu medicine.drug_class Breast Neoplasms DNA Fragmentation Biology Monoclonal antibody breast cancer Tumor Cells Cultured medicine Humans Bcl-2 Dose-Response Relationship Drug Cell Cycle apoptosis Wild type Antibodies Monoclonal Regular Article DNA Neoplasm Cell cycle Proto-Oncogene Proteins c-bcl-2 Oncology Cell culture Apoptosis Cancer research biology.protein Tumor Suppressor Protein p53 Antibody Cell Division |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1054/bjoc.2001.2197 |
| Popis: | Anti-Her-2/neu antibody is known to induce apoptosis in HER-2/neu overexpressing breast cancer cells. However, exact regulatory mechanisms mediating and controlling this phenomenon are still unknown. In the present study, we have investigated the effect of anti-Her-2/neu antibody on apoptosis of HER-2/neu overexpressing human breast cancer cell lines SK-BR-3, HTB-24, HTB-25, HTB-27, HTB-128, HTB-130 and HTB-131 in relation to p53 genotype and bcl-2 status. SK-BR-3, HTB-24, HTB-128 and HTB-130 cells exhibited mutant p53, whereas wild type p53 was found in HTB-25, HTB-27 and HTB-131 cells. All seven cell lines weakly expressed bcl-2 protein (10–20%). Anti-Her-2/neu antibody, irrespective of p53 and bcl-2 status, induced apoptosis in all 7 cell lines dose- and time-dependently and correlated with Her-2/neu overexpression. In addition, incubation of cell lines with anti-Her-2/neu antibody did not alter p53 or bcl-2 expression. Anti-HER-2/neu antibody did not induce apoptosis in HER-2/neu negative HBL-100 and HTB-132 cell lines. Our results indicate that within the panel of tested breast cancer cell lines, anti-Her-2/neu antibody-induced apoptosis was independent from the presence of intact p53. © 2001 Cancer Research Compaign http://www.bjcancer.com |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|