Modulation of the Dental Pulp Stem Cell Secretory Profile by Hypoxia Induction Using Cobalt Chloride
| Autor: | Nishant Vyas, Shilpa Bhandi, Luca Testarelli, Ahmed Al Kahtani, Satish Vishwanathaiah, Shankargouda Patil, Vikrant R Patil, Andrea Del Giudice, Loai Alsofi, Prabhadevi C Maganur, Mohammed Mashyakhy, A. Thirumal Raj, Deepak Mehta |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
hypoxia inducible factor lcsh:Medicine Medicine (miscellaneous) Article stemness 03 medical and health sciences Paracrine signalling 0302 clinical medicine stomatognathic system SOX2 Dental pulp stem cells Gene expression Viability assay Transcription factor Chemistry secretory profile cobalt chloride dental pulp stem cells lcsh:R 030206 dentistry Cell biology 030104 developmental biology Hypoxia-inducible factors Stem cell |
| Zdroj: | Journal of Personalized Medicine Volume 11 Issue 4 Journal of Personalized Medicine, Vol 11, Iss 247, p 247 (2021) |
| ISSN: | 2075-4426 |
| DOI: | 10.3390/jpm11040247 |
| Popis: | The action of stem cells is mediated by their paracrine secretions which comprise the secretory profile. Various approaches can be used to modify the secretory profile of stem cells. Creating a hypoxic environment is one method. The present study aims to demonstrate the influence of CoCl2 in generating hypoxic conditions in a dental pulp stem cell (DPSCs) culture, and the effect of this environment on their secretory profile. DPSCs that were isolated from human permanent teeth were characterized and treated with different concentrations of CoCl2 to assess their viability by an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and proliferation by a cell counting kit (CCK)-8 assay. The gene expression level of hypoxia-inducible factor 1-alpha (HIF-1α) was analyzed by quantitative real time polymerase chain reaction (qRT-PCR) to demonstrate a hypoxic environment. Comparative evaluation of the growth factors and cytokines were done by cytometric bead array. Gene expression levels of transcription factors OCT4 and SOX2 were analyzed by qRT-PCR to understand the effect of CoCl2 on stemness in DPSCs. DPSCs were positive for MSC-specific markers. Doses of CoCl2, up to 20 µM, did not negatively affect cell viability in low doses (5 µM), it promoted cell survival. Treatment with 10 µM of CoCl2 significantly augmented the genetic expression of HIF-1α. Cells treated with 10 µM of CoCl2 showed changes in the levels of growth factors and cytokines produced. It was very evident that CoCl2 also increased the expression of OCT4 and SOX2, which is the modulation of stemness of DPSCs. A CoCl2 treatment-induced hypoxic environment modulates the secretory profile of DPSCs. |
| Databáze: | OpenAIRE |
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