Neural crest derivatives and neuroendocrine cells in the gut of anencephalic and fetuses without congenital defects
| Autor: | Ricardo Fierro Murga, Dora Virginia Chávez-Corral, Hugo Alberto Cervantes-Flores, Samuel Bencomo-Loeppky, Margarita Levario-Carrillo, Sandra Reza-López, Martha Elena Loya-Loya, Jorge Arturo García-Rizk |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Embryology Pathology medicine.medical_specialty Health Toxicology and Mutagenesis Organogenesis Enteroendocrine cell 030105 genetics & heredity Biology Toxicology Enteric Nervous System 03 medical and health sciences symbols.namesake Fetus Neuroendocrine Cells medicine Humans Neural tube Neural crest Chromogranin A Interstitial cell of Cajal 030104 developmental biology medicine.anatomical_structure Neural Crest Peripheral nervous system Pediatrics Perinatology and Child Health symbols biology.protein Synaptophysin Enteric nervous system Female Developmental Biology |
| Zdroj: | Birth defects researchREFERENCES. 112(19) |
| ISSN: | 2472-1727 |
| Popis: | BACKGROUND The enteric nervous system (ENS), a component of the peripheral nervous system in the intestinal walls, regulates motility, secretion, absorption, and blood flow. Neural crest (NC) migration, fundamental for ENS development, may be altered by central nervous system development alterations, such as neural tube defects (NTD). Intestinal innervation anomalies have been correlated to NTD. We aim to describe the ENS on a fetus with NTD and fetuses without congenital defects (FWCD). CASES Two male and four female FWCD, 18-20 weeks-gestation (WG), and a 25 WG female anencephalic fetus. Samples from the pancreatoduodenal groove, jejunum, cecum, rectum, and appendix were analyzed by immunohistochemistry. Nervous plexuses were marked with Neuron-specific enolase and S-100; enteric glial cells with CD56; neuroendocrine cells with chromogranin and synaptophysin, and interstitial cells of Cajal (ICC) with CD117. RESULTS AND CONCLUSION The anencephalic fetus presented a rudimentary brainstem with a cerebellum. Partial frontal, temporal, and occipital bones were found. A large atrial septal defect, an enlarged kidney with a duplex collecting system and a single adrenal gland were found. NSE, S100, and CD56, showed the presence of the myenteric and submucous plexuses of the ENS; scarce interplexus reactivity may indicate inadequate development. Pancreatic and gut neuroendocrine cells, identified with chromogranin and CD56, showed that the enteroendocrine system is present. Findings on FWCD using these markers are consistent with literature descriptions. Vagal NC migration appears to be unaffected despite the presence of anencephaly, although maturation of the ENS may be altered. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text