Cytosolic superoxide dismutase can provide protection against Fasciola gigantica
| Autor: | Narin Changklungmoa, Rataya Tanomrat, Kulathida Chaithirayanon, Prasert Sobhon, Pornanan Kueakhai, Wipaphorn Jaikua, Sirima Wongwairot, Suda Riengrojpitak |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Fascioliasis Veterinary (miscellaneous) Fasciola gigantica Freund's Adjuvant Antibodies Helminth Cross Reactions Microbiology Superoxide dismutase 03 medical and health sciences Mice Cytosol Antigen Animals Humans Metacercariae Paramphistomum cervi Antiserum chemistry.chemical_classification Reactive oxygen species Fasciola biology Superoxide Dismutase 030108 mycology & parasitology biology.organism_classification Virology Recombinant Proteins Infectious Diseases chemistry Polyclonal antibodies Insect Science Immunoglobulin G biology.protein Parasitology Rabbits |
| Zdroj: | Acta tropica. 162 |
| ISSN: | 1873-6254 |
| Popis: | Superoxide dismutases (SOD), antioxidant metallo-enzymes, are a part of the first line of defense in the trematode parasites which act as the chief scavengers for reactive oxygen species (ROS). A recombinant Fasciola gigantica cytosolic SOD (FgSOD) was expressed in Escherichia coli BL21 (DE3) and used for immunizing rabbits to obtain polyclonal antibodies (anti-rFgSOD). This rabbit anti-rFgSOD reacted with the native FgSOD at a molecular weight of 17.5kDa. The FgSOD protein was expressed at high level in parenchyma, caecal epithelium and egg of the parasite. The rFgSOD reacted with antisera from rabbits infected with F. gigantica metacercariae collected at 2, 5, and 7 weeks after infection, and reacted with sera of infected mice. Anti-rFgSOD exhibited cross reactivity with the other parasites' antigens, including Eurytrema pancreaticum, Cotylophoron cotylophorum, Fischoederius cobboldi, Gastrothylax crumenifer, Paramphistomum cervi, and Setaria labiato papillosa. A vaccination was performed in imprinting control region (ICR) mice by subcutaneous injection with 50μg of rFgSOD combined with Freund's adjuvant. At 2 weeks after the second boost, mice were infected with 15 metacercariae by oral route. IgG1 and IgG2a in the immune sera were determined to indicate Th2 and Th1 immune responses. It was found that the parasite burden was reduced by 45%, and both IgG1 and IgG2a levels showed correlation with the numbers of worm recoveries. |
| Databáze: | OpenAIRE |
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