A novel monoclonal antibody specific for the N-terminal end of GAD65
| Autor: | Terri Daniels, Santica M. Marcovina, Åke Lernmark, Lisa P. Hammerle, Per Lundgren, Christiane S. Hampe |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Gene isoform
endocrine system DNA Complementary endocrine system diseases medicine.drug_class Blotting Western Molecular Sequence Data Immunology Immunocytochemistry Radioimmunoassay Immunoglobulin light chain Monoclonal antibody Mice Antibody Specificity immune system diseases medicine Animals Immunology and Allergy Amino Acid Sequence Peptide sequence Autoantibodies Mice Inbred BALB C biology Glutamate Decarboxylase Reverse Transcriptase Polymerase Chain Reaction Nucleic acid sequence Antibodies Monoclonal nutritional and metabolic diseases Immunohistochemistry Molecular biology Protein Structure Tertiary Isoenzymes Diabetes Mellitus Type 1 Neurology Biochemistry GenBank biology.protein Immunoglobulin Light Chains Neurology (clinical) Antibody Immunoglobulin Heavy Chains |
| Zdroj: | Journal of Neuroimmunology. 113:63-71 |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/s0165-5728(00)00423-9 |
| Popis: | We describe a novel monoclonal antibody raised towards the N-terminus of the 65 kDa isoform of glutamate decarboxylase (GAD65). This N-GAD65 mAb is highly specific for GAD65 and can be used in a wide range of applications such as Western blot analysis, immunoprecipitation and immunocytochemistry. Full-length cDNAs coding for N-GAD65 mAb heavy and light chains were cloned and characterized by nucleotide sequence analysis. Both heavy and light chain-specific cDNAs are functional and are members of mouse heavy chain subgroup IgG1 and kappa chain group 2, respectively. Comparing the N-GAD65 mAb gene sequences with GenBank shows that the cDNAs were not reported previously. |
| Databáze: | OpenAIRE |
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