Association of a pool of HIV-1 with erythrocytes in vivo: a cohort study

Autor:	J A Schifferli, Manuel Battegay, Salima Sadallah, Luregn J. Schlapbach, Thomas Klimkait, Christoph Schaefer, Verena Werder, Christoph Hess, Eliska Horakova, Veronika Del Zenero, Gianmarco M. Balestra
Rok vydání:	2002
Předmět:	Adult Male Erythrocytes HIV Infections Virus Cohort Studies Immune system Antiretroviral Therapy Highly Active medicine Humans biology Reverse Transcriptase Polymerase Chain Reaction virus diseases RNA General Medicine biology.organism_classification Virology Red blood cell medicine.anatomical_structure Viral replication Lentivirus Immunology HIV-1 RNA Viral Female Viral disease Viral load
Zdroj:	The Lancet. 359:2230-2234
ISSN:	0140-6736
Popis:	Treatment of HIV-1-infected individuals with antiretrovirals can result in sustained suppression of plasma viral RNA at concentrations below the detection limit of available assays. However, continuing virus replication has been detected in patients with viral RNA in plasma suppressed for months to years, and many cell types are known to act as reservoirs or carriers for the virus. In vitro, erythrocytes bind HIV-1 immune complexes, so we tested for a circulating pool of HIV-1 associated with erythrocytes in people with HIV-1 infection.We investigated 82 chronically HIV-1-infected individuals. Plasma, white cells, and erythrocytes were tested for HIV-1 RNA by RT-PCR.Erythrocyte-associated HIV-1 RNA was detected in 80 of 82 individuals. In 23, plasma HIV-1 RNA had been undetectable (20 copies/mL) for up to 32 months; in corresponding erythrocyte samples, there were up to 82878 HIV-1 RNA copies per mL whole blood. HIV-1 associated with erythrocytes in vivo was shown to be infectious. Within the subgroup of patients with undetectable plasma viral load, higher numbers of HIV-1 associated with erythrocytes were correlated with a history of advanced clinical stages of HIV-1 infection (p=0.014).A pool of HIV-1 is associated with erythrocytes even after long-term suppression of viral RNA in plasma. This finding is direct evidence for continuing virus replication or release in these individuals. Quantification of this viral pool may help to judge suppression of HIV-1 replication in individuals with undetectable plasma HIV-1 RNA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ac5e453605d1251801f75a97daebc27 https://doi.org/10.1016/s0140-6736(02)09291-7 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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