RGD-mediated adhesion of porcine granulosa cells modulates their differentiation response to FSH in vitro

Autor: Jacques E. Fléchon, Roland Salesse, Solange Delasalle, Béatrice Goxe
Přispěvatelé: ProdInra, Migration, Unité de biologie cellulaire et moléculaire, Institut National de la Recherche Agronomique (INRA)
Rok vydání: 1995
Předmět:
endocrine system
medicine.medical_specialty
Integrins
medicine.drug_class
Swine
[SDV]Life Sciences [q-bio]
Receptor expression
Molecular Sequence Data
030209 endocrinology & metabolism
Biology
In Vitro Techniques
03 medical and health sciences
Follicle-stimulating hormone
0302 clinical medicine
Aromatase
Internal medicine
FSH
medicine
Extracellular
Animals
Amino Acid Sequence
ComputingMilieux_MISCELLANEOUS
Cells
Cultured
Progesterone
030304 developmental biology
0303 health sciences
Granulosa Cells
Cell Differentiation
Cell Biology
General Medicine
Receptors
LH
Urokinase-Type Plasminogen Activator
Actins
Fibronectins
[SDV] Life Sciences [q-bio]
Fibronectin
Actin Cytoskeleton
Endocrinology
Estrogen
Cell culture
biology.protein
Female
Gonadotropin
Follicle Stimulating Hormone
Luteinizing hormone
Peptides
Cell Adhesion Molecules
Oligopeptides
Antimicrobial Cationic Peptides
Zdroj: Biology of the Cell
Biology of the Cell, Wiley, 1995, 83, pp.169-177
ISSN: 0248-4900
1768-322X
Popis: Porcine granulosa cells cultured in serum-free medium undergo metabolic and morphologic changes after follicle stimulating hormone (FSH) stimulation. Under these conditions, granulosa cells differentiate and tend to round up and their links with the plastic support are reduced. Coating of culture substratum with PepTite-2000, an integrin-binding synthetic peptide containing RGD (Arg-Gly-Asp) sequences enhanced the plating of granulosa cells. Whether the peptide be present or not, cells cultivated in basal synthetic medium (without FSH) were flattened and attached to the substratum by stress fibers at focal contacts where integrin beta 1, extracellular fibronectin, and urokinase plasminogen activator colocalized. After FSH stimulation, part of the cells rounded up and F-actin took a more uniform, cortical localization. Correlatively, extracellular fibronectin aggregated in a clump, while integrin beta 1 and urokinase plasminogen activator spread over rounded cells. These morphological changes elicited by FSH were little affected by the presence of PepTite-2000, yet a larger number of cells remained flattened. However, concerning steroidogenesis, increasing concentrations of peptide seemed to favor progesterone rather than estrogen production, and to restrain luteinizing hormone (LH) receptor expression, suggesting a premature commitment of cells towards luteinization rather than completion of follicular preovulatory differentiation.
Databáze: OpenAIRE
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