The Ambivalent Role of Glutathione in the Protection of Dna Against Singlet Oxygen

Autor:	Hans Joenje, M.V.M. Lafleur, E.J. Westmijze, J. J. Hoorweg, J. Retèl
Rok vydání:	1994
Předmět:	Azides Chemistry Singlet oxygen DNA Single-Stranded General Medicine Glutathione Biochemistry Oxygen chemistry.chemical_compound DNA Viral Sodium Azide DNA DNA Damage Mutagens
Zdroj:	Free Radical Research. 21:9-17
ISSN:	1029-2470 1071-5762
Popis:	Glutathione (GSH) was examined with respect to its ability to protect DNA against 1O2 damage. We have found that GSH protected, at least partly, the DNA against inactivation by 1O2. Up to 10 mM the protection increased as a function of GSH concentration. Above 10 mM the protection remained constant and less than expected on the basis of scavenging/quenching of 1O2, in contrast to the protection offered by sodium-azide. Especially at the higher concentrations of GSH the protection against the biological inactivation is accompanied by an increase in single-strand breaks and also probably lethal base damage. However, all together the data suggest that at least in the physiologically important range (0.1-10 mM) GSH is able to protect efficiently against 1O2-induced inactivating DNA damage.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ac37249f746b6739654e1b3f5e38f54 https://doi.org/10.3109/10715769409056550 Zobrazit plný text záznamu