Single molecule detection and photochemistry on a surface using near-field optical excitation

Autor:	John C. Martin, Peter M. Goodwin, Richard A. Keller, W.P. Ambrose
Rok vydání:	1994
Předmět:	Materials science genetic structures General Physics and Astronomy Photochemistry Fluorescence Photobleaching Fluorescence spectroscopy law.invention Rhodamine 6G chemistry.chemical_compound chemistry Optical microscope law Microscopy Excitation Fluorescence loss in photobleaching
Zdroj:	Physical Review Letters. 72:160-163
ISSN:	0031-9007
DOI:	10.1103/physrevlett.72.160
Popis:	We report the first photobleaching experiments of single molecules on a surface. Single molecules of rhodamine 6G are detected on a silica surface under ambient conditions using near-field scanning optical microscopy. Photobleaching of an individual molecule appears as an abrupt disappearance of fluorescence after seconds of constant excitation. Other reversible, upward and downward changes in fluorescence are observed, possibly due to reorientation of molecules on the surface.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ac297a483132d62928b6fcc0bc58a58 https://doi.org/10.1103/physrevlett.72.160 Zobrazit plný text záznamu