Mouse embryonic stem cells maintain differentiation potency into somatic lineage despite alternation of ploidy
| Autor: | Hiroyuki Imai, Wataru Fujii, Ken Takeshi Kusakabe, Yasuo Kiso, Kiyoshi Kano |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Zygote. 30:480-486 |
| ISSN: | 1469-8730 0967-1994 |
| Popis: | SummaryVertebrates, including mammals, are considered to have evolved by whole genome duplications. Although some fish have been reported to be polyploids that have undergone additional genome duplication, there have been no reports of polyploid mammals due to abnormal development after implantation. Furthermore, as the number of physiologically existing tetraploid somatic cells is small, details of the functions of these ploidy-altered cells are not fully understood. In this present study, we aimed to clarify the details of the differentiation potency of tetraploids using tetraploid embryonic stem cells. To clarify the differentiation potency, we used mouse tetraploid embryonic stem cells derived from tetraploid embryos. We presented tetraploid embryonic stem cells differentiated into neural and osteocyte lineage in vitro and tetraploid cells that contributed to various tissues of chimeric embryos ubiquitously in vivo. These results revealed that mouse embryonic stem cells maintain differentiation potency after altering the ploidy. Our results provide an important basis for the differentiation dynamics of germ layers in mammalian polyploid embryogenesis. |
| Databáze: | OpenAIRE |
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