Fungal feruloyl esterases: Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies
| Autor: | Dilokpimol, Adiphol, Mäkelä, Miia R, Varriale, Simona, Zhou, Miaomiao, Cerullo, Gabriella, Gidijala, Loknath, Hinkka, Harri, Brás, Joana L A, Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Hildén, Kristiina S, Faraco, Vincenza, de Vries, Ronald P, Sub Molecular Plant Physiology, Molecular Plant Physiology |
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| Přispěvatelé: | Department of Food and Nutrition, Fungal Genetics and Biotechnology, Department of Microbiology, Helsinki Institute of Sustainability Science (HELSUS), Sub Molecular Plant Physiology, Molecular Plant Physiology, Westerdijk Fungal Biodiversity Institute, Westerdijk Fungal Biodiversity Institute - Fungal Physiology, Dilokpimol, Adiphol, Mäkelä, Miia R., Varriale, Simona, Zhou, Miaomiao, Cerullo, Gabriella, Gidijala, Loknath, Hinkka, Harri, Brás, Joana L. A., Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Hildén, Kristiina S., Faraco, Vincenza, de Vries, Ronald P. |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine 01 natural sciences Substrate Specificity law.invention Ferulic acid chemistry.chemical_compound Feruloyl esterase law Genome mining Data Mining CRYSTAL-STRUCTURE SPECIFICITY chemistry.chemical_classification Genome biology Fungi/enzymology General Medicine Carboxylic Ester Hydrolases/genetics Recombinant Proteins FAMILY OLIGOSACCHARIDES Fungal Biochemistry ACID Recombinant DNA Genome Fungal Biotechnology ASPERGILLUS-NIGER Bioengineering Pichia pastoris Cell wall 03 medical and health sciences Hydrolysis 010608 biotechnology Plant cell wall Recombinant Proteins/biosynthesis Molecular Biology PURIFICATION Aspergillus niger Fungi Reproducibility of Results DEGRADATION PERFORMANCE biology.organism_classification Molecular Weight 030104 developmental biology Enzyme chemistry 1182 Biochemistry cell and molecular biology Carboxylic Ester Hydrolases CELL WALL POLYSACCHARIDES |
| Zdroj: | New Biotechnology, 41, 9. Elsevier New Biotechnology, 41, 9-14. Elsevier B.V. Dilokpimol, A, Mäkelä, M R, Varriale, S, Zhou, M, Cerullo, G, Gidijala, L, Hinkka, H, Brás, J L A, Jütten, P, Piechot, A, Verhaert, R, Hildén, K S, Faraco, V & de Vries, R P 2018, ' Fungal feruloyl esterases : Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies ', New Biotechnology, vol. 41, pp. 9-14 . https://doi.org/10.1016/j.nbt.2017.11.004 |
| ISSN: | 1871-6784 |
| DOI: | 10.1016/j.nbt.2017.11.004 |
| Popis: | Feruloyl esterases (FAEs) are a diverse group of enzymes that specifically catalyze the hydrolysis of ester bonds between a hydroxycinnamic (e.g. ferulic) acid and plant poly- or oligosaccharides. FAEs as auxiliary enzymes significantly assist xylanolytic and pectinolytic enzymes in gaining access to their site of action during biomass saccharification for biofuel and biochemical production. A limited number of FAEs have been functionally characterized compared to over 1000 putative fungal FAEs that were recently predicted by similarity-based genome mining, which divided phylogenetically into different subfamilies (SFs). In this study, 27 putative and six characterized FAEs from both ascomycete and basidiomycete fungi were selected and heterologously expressed in Pichia pastoris and the recombinant proteins biochemically characterized to validate the previous genome mining and phylogenetical grouping and to expand the information on activity of fungal FAEs. As a result, 20 enzymes were shown to possess FAE activity, being active towards pNP-ferulate and/or methyl hydroxycinnamate substrates, and covering 11 subfamilies. Most of the new FAEs showed activities comparable to those of previously characterized fungal FAEs. |
| Databáze: | OpenAIRE |
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