Expansion within the CYP71D subfamily drives the heterocyclization of tanshinones synthesis in Salvia miltiorrhiza
| Autor: | Jian Yang, Yanan Wang, Chang-Jiang-Sheng Lai, Luqi Huang, Li-Ping Kang, Wen Zeng, Tong Chen, Ye Shen, Baolong Jin, Xiaoquan Qi, Xiaohui Ma, Yujun Zhao, Reuben J. Peters, Jinfu Tang, Guanghong Cui, Rui-Shan Wang, Juan Guo, Ying Ma |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Subfamily Science General Physics and Astronomy Salvia miltiorrhiza Salvia Genes Plant Plant Roots 01 natural sciences Article General Biochemistry Genetics and Molecular Biology Evolution Molecular Hydroxylation Plant evolution 03 medical and health sciences chemistry.chemical_compound Cytochrome P-450 Enzyme System Gene cluster Secondary metabolism Plant Proteins Multidisciplinary biology fungi Cytochrome P450 food and beverages Genomics General Chemistry biology.organism_classification 030104 developmental biology Biochemistry chemistry Cyclization Multigene Family Abietanes biology.protein Lamiaceae Genome Plant Drugs Chinese Herbal 010606 plant biology & botany |
| Zdroj: | Nature Communications, Vol 12, Iss 1, Pp 1-11 (2021) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Tanshinones are the bioactive nor-diterpenoid constituents of the Chinese medicinal herb Danshen (Salvia miltiorrhiza). These groups of chemicals have the characteristic furan D-ring, which differentiates them from the phenolic abietane-type diterpenoids frequently found in the Lamiaceae family. However, how the 14,16-epoxy is formed has not been elucidated. Here, we report an improved genome assembly of Danshen using a highly homozygous genotype. We identify a cytochrome P450 (CYP71D) tandem gene array through gene expansion analysis. We show that CYP71D373 and CYP71D375 catalyze hydroxylation at carbon-16 (C16) and 14,16-ether (hetero)cyclization to form the D-ring, whereas CYP71D411 catalyzes upstream hydroxylation at C20. In addition, we discover a large biosynthetic gene cluster associated with tanshinone production. Collinearity analysis indicates a more specific origin of tanshinones in Salvia genus. It illustrates the evolutionary origin of abietane-type diterpenoids and those with a furan D-ring in Lamiaceae. Salvia miltiorrhiza is a medicinal plant that can produce the bioactive tanshinones. Here, the authors report the improved genome assembly and reveal the possible roles of three CYP71Ds in catalyzing the reactions leading to the formation of the characteristic furan D-ring of transhinones. |
| Databáze: | OpenAIRE |
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