Detection and identification of Chlamydia spp. from pigeons in Iran by nested PCR and sequencing
| Autor: | Payam Haghighi Khoshkhoo, Nadia Golestani, Gita Akbari Azad, Hossein Hosseini |
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| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Serotype Pigeons lcsh:QR1-502 Biology 01 natural sciences Microbiology Psittacosis lcsh:Microbiology law.invention law 0502 economics and business Genotype medicine Chlamydia avium Genotype B 0101 mathematics Polymerase chain reaction Chlamydia psittaci Chlamydia Phylogenetic tree 010102 general mathematics 05 social sciences bacterial infections and mycoses medicine.disease biology.organism_classification Virology Nested polymerase chain reaction psychological phenomena and processes 050203 business & management |
| Zdroj: | Iranian Journal of Microbiology, Vol 12, Iss 4 (2020) |
| ISSN: | 2008-4447 2008-3289 |
| DOI: | 10.18502/ijm.v12i4.3937 |
| Popis: | Background and Objectives: Chlamydia psittaci, an obligate intracellular, Gram-negative zoonotic pathogen, has eight serovars and nine genotypes isolated from avian species with higher frequency in parrots and pigeons. The aim of this study was to characterize Chlamydia spp. using nested PCR and sequencing. Materials and Methods: A total of 270 pharyngeal swab samples collected randomly from asymptomatic pigeons of 30 pigeon aviaries in Tehran province. DNA was extracted with specific kit and amplified by specific primers in the first PCR and outer membrane protein A (ompA) gene in the second PCR. Positive samples were sequenced and phylogenetic tree analyzed based on the ompA gene. Results: Records showed that 16 of 30 (53%) pigeon aviaries were positive for Chlamydia spp. Phylogenetic tree analysis revealed that 15 of 16 (93.7%) positive samples, belonged to C. psittaci genotype B whereas the other sample belonged to C. avium. C. psittaci detected in 50% of pigeon aviaries that is high rate in Tehran province. Conclusion: As C. psittaci is a zoonosis and life threaten pathogen for human being, these results indicate the significance of it detection in asymptomatic pigeons. Also, this is the first report of Chlamydia avium presence in Iranian pigeons which its zoonotic potential is still unknown. |
| Databáze: | OpenAIRE |
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