Grandisin induces apoptosis in leukemic K562 cells
| Autor: | Elizabeth Gomes Paulino Menezes, Marize Campos Valadares, Eliana Martins Lima, Alane Pereira Cortez, Alexandre Pereira dos Santos, Higor de Oliveira Ribeiro, Larissa Moreira Cleres, Polyana Lopes Benfica, Massuo J. Kato |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Lignan Leukemic lcsh:RS1-441 Cell cycle Biology Molecular biology Cell biology lcsh:Pharmacy and materia medica 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine chemistry Cell Death Process Apoptosis Grandisin/antileukemic activities 030220 oncology & carcinogenesis Caspase activation Trypan blue General Pharmacology Toxicology and Pharmaceutics K562 Cytotoxicity K562 cells |
| Zdroj: | Brazilian Journal of Pharmaceutical Sciences; Vol. 53 No. 1 (2017); e15210 Brazilian Journal of Pharmaceutical Sciences; Vol. 53 Núm. 1 (2017); e15210 Brazilian Journal of Pharmaceutical Sciences; v. 53 n. 1 (2017); e15210 Brazilian Journal of Pharmaceutical Sciences Universidade de São Paulo (USP) instacron:USP Brazilian Journal of Pharmaceutical Sciences, Vol 53, Iss 1 (2017) Brazilian Journal of Pharmaceutical Sciences, Volume: 53, Issue: 1, Article number: e15210, Published: 20 APR 2017 |
| ISSN: | 2175-9790 1984-8250 |
| Popis: | In this study, the potential antileukemic activity of grandisin, a lignan extracted from Piper solmsianum, was evaluated against the leukemic line K562. The cytotoxicity of grandisin (0.018 to 2.365 µM) was evaluated in K562 and normal peripheral blood lymphocytes by Trypan Blue Exclusion and MTT methods after 48h exposure to the drug. In both methods, cellular viability was concentration-dependent and the IC50 values were lower than 0.85µM. Analysis of K562 cells after treatment with grandisin showed that the cell cycle was arrested in the G1 phase with a 12.31% increase, while both S and G2 phases decreased. Morphological studies conducted after the exposure of K562 to grandisin revealed changes consistent with the apoptosis process, which was confirmed by anexin V stain and caspase activation. Thus, lignan grandisin showed antileukemic activities against the K562 cell line and the cell death process occurred via apoptosis. |
| Databáze: | OpenAIRE |
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