An inter-laboratory comparison of urinary 3-hydroxypropylmercapturic acid measurement demonstrates good reproducibility between laboratories
| Autor: | Emmanuel Minet, Mike McEwan, Graham Errington, Kirk Newland, Francis Cheung, Brian Bailey, Gerhard Scherer, Mehran Sharifi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
3-Hydroxypropylmercapturic Acid
Urinary system Metabolite Short Report lcsh:Medicine Tandem mass spectrometry Bioinformatics General Biochemistry Genetics and Molecular Biology Tobacco smoke chemistry.chemical_compound Medicine lcsh:Science (General) lcsh:QH301-705.5 Medicine(all) Reproducibility Chromatography Biochemistry Genetics and Molecular Biology(all) business.industry lcsh:R General Medicine chemistry lcsh:Biology (General) Biomarker (medicine) business Toxicant lcsh:Q1-390 |
| Zdroj: | BMC Research Notes, Vol 4, Iss 1, p 391 (2011) BMC Research Notes |
| ISSN: | 1756-0500 |
| Popis: | Background Biomarkers have been used extensively in clinical studies to assess toxicant exposure in smokers and non-smokers and have recently been used in the evaluation of novel tobacco products. The urinary metabolite 3-HPMA, a metabolite of the major tobacco smoke toxicity contributor acrolein, is one example of a biomarker used to measure exposure to tobacco smoke. A number of laboratories have developed liquid chromatography with tandem mass spectrometry (LC-MS/MS) based methods to measure urinary 3-HPMA; however, it is unclear to what extent the data obtained by these different laboratories are comparable. Findings This report describes an inter-laboratory comparison carried out to evaluate the comparability of 3-HPMA measurement between four laboratories. A common set of spiked and authentic smoker and non-smoker urine samples were used. Each laboratory used their in-house LC-MS/MS method and a common internal standard. A comparison of the repeatability ('r'), reproducibility ('R'), and coefficient of variation for 3-HPMA demonstrated that within-laboratory variation was consistently lower than between-laboratory variation. The average inter-laboratory coefficient of variation was 7% for fortified urine samples and 16.2% for authentic urine samples. Together, this represents an inter-laboratory variation of 12.2%. Conclusion The results from this first inter-laboratory comparison for the measurement of 3-HPMA in urine demonstrate a reasonably good consensus between laboratories. However, some consistent measurement biases were still observed between laboratories, suggesting that additional work may be required to further reduce the inter-laboratory coefficient of variation. |
| Databáze: | OpenAIRE |
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