Ixodid Tick Dissection and Tick Ex Vivo Organ Cultures for Tick‐Borne Virus Research
| Autor: | Ryan Kissinger, Jeffrey M. Grabowski |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Disease Biology Tick Microbiology Article Salivary Glands Virus 03 medical and health sciences Organ Culture Techniques RNA interference Virology parasitic diseases medicine Animals Pathogen 030304 developmental biology 0303 health sciences Ixodes Salivary gland 030306 microbiology Dissection bacterial infections and mycoses biology.organism_classification medicine.anatomical_structure Tick-Borne Diseases Ixodes scapularis Female RNA Interference Parasitology Ex vivo |
| Zdroj: | Curr Protoc Microbiol |
| ISSN: | 1934-8533 1934-8525 |
| DOI: | 10.1002/cpmc.118 |
| Popis: | Tick-borne viruses cause thousands of cases of disease worldwide every year. Specific countermeasures to many tick-borne viruses are not commercially available. Very little is known regarding tick-virus interactions and increasing this knowledge can lead to potential targets for countermeasure development. Virus infection of ex vivo organ cultures from ticks can provide an approach to identify susceptible cell types of tissue to infection. Additionally, these organ cultures can be used for functional genomic studies to pinpoint tick-specific genes involved in the virus lifecycle. Provided here are step-by-step procedures to set up basic tick organ cultures in combination with virus infection and/or functional genomic studies. These procedures can be adapted for future use to characterize other tick-borne pathogen infections as well as tick-specific biological processes. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Loading 96-well plates with gelfoam substrate Basic Protocol 2: Step-by-step aseptic dissection of unfed female/male Ixodes scapularis ticks for multiple organs Basic Protocol 3: Step-by-step aseptic dissection of fed female Ixodes scapularis ticks to remove salivary glands Basic Protocol 4: Metabolic viability analyses of tick organ cultures Basic Protocol 5: Virus infection of tick organ cultures Basic Protocol 6: Functional RNA interference analyses using tick organ cultures. |
| Databáze: | OpenAIRE |
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