Enhancement of propyl gallate-induced calf pulmonary arterial endothelial cell death by MEK and JNK inhibitors

Autor:	Sung Z. Kim, Yong H. Han, Woo H. Park, Suhn Hee Kim
Rok vydání:	2009
Předmět:	MAPK/ERK pathway Cancer Research Programmed cell death Cell growth p38 mitogen-activated protein kinases Glutathione Pharmacology Biology Biochemistry Cell biology chemistry.chemical_compound Oncology chemistry Apoptosis Genetics Molecular Medicine Protein kinase A Molecular Biology Propyl gallate
Zdroj:	Molecular Medicine Reports. 2
ISSN:	1791-3004 1791-2997
DOI:	10.3892/mmr_00000179
Popis:	Propyl gallate (PG), a synthetic antioxidant, exerts a variety of effects on tissue and cell functions. Here, we investigated the effect of mitogen-activated protein kinase (MAPK) inhibitors on PG-treated calf pulmonary artery endothelial cells (CPAECs) in relation to changes in cell death, reactive oxygen species (ROS) and glutathione (GSH). PG inhibited CPAEC growth at 24 h and induced cell death, which was accompanied by the loss of mitochondrial membrane potential. PG also increased ROS levels in the CPAECs, while GSH depleted cell number. Treatment with MAPK (MEK, JNK and p38) inhibitors resulted in the slight enhancement of cell growth inhibition by PG. MEK and JNK inhibitors increased cell death and GSH depletion in PG-treated CPAECs without affecting ROS levels. In conclusion, PG inhibited the growth of CPAECs by regulating GSH levels. The pro-apoptotic effect of MEK and JNK inhibitors on PG-induced CPAEC death was related to a decrease in GSH levels.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ab3fac48e9304bcb0ecdf8238b0918c https://doi.org/10.3892/mmr_00000179 Zobrazit plný text záznamu